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Merck
CN

69198

Fraser Broth, Base

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Listeria Selective Enrichement Broth, Base (acc. Fraser)

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171606

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Product Name

Fraser Broth, Base, suitable for microbiology, NutriSelect® Plus

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

casein enzymic hydrolysate, 5.0 g/L
disodium phosphate dihydrate, 12.0 g/L
esculin, 1.0 g/L
lithium chloride, 3.0 g/L
meat extract, 5.0 g/L
peptic digest of animal tissue, 5.0 g/L
potassium dihydrogen phosphate, 1.35 g/L
sodium chloride, 20.0 g/L
yeast extract, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.2±0.2 (25 °C)

application(s)

agriculture
environmental
food and beverages
pathogen testing
sample preparation
microbiology

suitability

selective and differential for Listeria spp.

Quality Level

200

Related Categories

Application

Fraser Broth Base with added supplement is recommended by ISO Committee for primary and secondary enrichment medium, for the isolation and enumeration of Listeria monocytogenes from food and animal feeds

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Suspend 57.35 grams of dehydrated medium in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Fraser Selective Supplement (Cat. No. 18038) and 2 vials of Fraser Supplement (Cat. No. 90836) to 1000 ml medium for primary enrichment or 1 vial of each to 500 ml medium for secondary enrichment. Mix well and dispense as desired.
Warning : Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin wash with plenty of water immediately.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN8240
BCCF3315
BCCD0292
BCCB7208
BCBZ9590

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