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68109

Millipore

Nickels and Leesment ChromoSelect Medium

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
Nickels and Leesment Medium + X-gal
UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
calcium lactate pentahydrate, 8.0 g/L
carboxymethyl cellulose (CMC), 0.40 g/L
casein enzymic hdrolysate, 18.0 g/L
chromogenic substrate (X-gal), 0.20 g/L
gelatine, 2.25 g/L
glucose, 4.5 g/L
lactose, 4.5 g/L
sodium chloride, 3.6 g/L
tricalcium dicitrate tetrahydrate, 6.65 g/L
trisodium citrate dihydrate, 1.8 g/L
yeast extract, 4.5 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

6.65±0.05 (25 °C)

application(s)

bioburden testing
environmental
food and beverages
microbiology

storage temp.

2-8°C

suitability

Lactococcus spp.
Leuconostoc spp.

Related Categories

Application

Nickels and Leesment ChromoSelect Medium is used for the enumeration of citrate-fermenting lactic acid bacteria from milk, milk products and mesophilic starter cultures.

Preparation Note

Supplement:
2 vl/L of Nickels and Leesment ChromoSelect Selective Supplement (Cat. No. 40952).
Suspend 66.0 grams of dehydrated medium in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired, add rehydrated contents of 2 vials of Nickels and Leesment ChromoSelect Selective Supplement (Cat. No. 40952).

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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