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66720

Sigma-Aldrich

Methylene Blue hydrate

purum p.a., ≥97.0% (calc. based on dry substance, AT)

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CN¥4,778.98

About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S · xH2O
CAS Number:
122965-43-9
Molecular Weight:
319.85 (anhydrous basis)
Beilstein:
3599847
EC Number:
200-515-2
MDL number:
MFCD00150006
UNSPSC Code:
12171500
PubChem Substance ID:
329760310
NACRES:
NA.32

CN¥4,778.98

Please contact Customer Service for Availability
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grade

purum p.a.

Quality Level

200

Assay

≥97.0% (calc. based on dry substance, AT)

suitability

suitable for microscopy

SMILES string

O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1

InChI key

WQVSELLRAGBDLX-UHFFFAOYSA-M

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General description

Methylene Blue (MB) is a basic[1] histological dye.[2]

Application

Methylene Blue hydrate has been used:
  • in the preparation of methylene blue (MB) solution to stain zymograms[1]
  • to label the injection site in the tissue slices[3]
  • to pre-treat mice via oral gavage to study its effects on noise-induced hearing loss[4]

Biochem/physiol Actions

Methylene blue (MB) plays a key role in microbiology and pharmacology. It is mainly used in staining live organisms and in methemoglobinemia treatment.[2] MB can be considered as a possible and safe vasoconstrictor.[5] It has several applications in textile, paper and hair coloring sectors.[1]

Other Notes

may contain up to 22% water
On the efficiency of methylene blue versus persulfate catalysis of polyacrylamide gels, as investigated by capillary zone electrophoresis[6]

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCN0560
    BCCM4231
    BCCH3435
    BCCK3438
    BCCD9107

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    Methylene blue in photodynamic therapy: from basic mechanisms to clinical applications
    Tardivo Jp, et al.
    Photodiagnosis and Photodynamic Therapy, 2(3), 175-191 (2005)
    Methylene blue in the treatment of vasodilatory shock: a Meta-analysis
    Zhang X, et al.
    Zhonghua Wei Zhong Bing ji Jiu yi Xue, 2(3), 175-191 (2017)
    Efficient removal of different basic dyes using graphene
    Iqbala MZ, et al.
    Desalination and Water Treatment, 68(3), 226-235 (2017)
    UDP-Induced Phagocytosis and ATP-Stimulated Chemotactic Migration Are Impaired in STIM1-/- Microglia In Vitro and In Vivo
    Lim HM, et al.
    Mediators of Inflammation, 2017(3), e1687-e1687 (2017)
    Attenuation of noise-induced hearing loss using methylene blue
    Park JS, et al.
    Cell Death & Disease, 5(4), e1200-e1200 (2014)
    View All Related Papers

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