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Merck
CN

66011

Alcianblue 8GX solution

suitable for microscopy, 1% in solution (in 3% acetic acid)

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About This Item

CAS Number:
75881-23-1
UNSPSC Code:
12171500
PubChem Substance ID:
329760145
NACRES:
MA.02
MDL number:
MFCD00010720

Key Documents

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Product Name

Alcianblue 8GX solution, suitable for microscopy, 1% in solution (in 3% acetic acid)

SMILES string

[Cu](Cl)Cl.S(Cc1cc2c3[n-]c(nc4nc(nc6nc(nc8[n-]c(n3)c9c8cc(cc9)CSC(=[N+](C)C)N(C)C)c7c6cc(cc7)CSC(=[N+](C)C)N(C)C)c5c4cc(cc5)CSC(=[N+](C)C)N(C)C)c2cc1)C(=[N+](C)C)N(C)C.[Cl-].[Cl-]

InChI

1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4

InChI key

CKLBXIYTBHXJEH-UHFFFAOYSA-J

form

liquid

concentration

1% in solution (in 3% acetic acid)

technique(s)

microbe id | staining: suitable

density

0.996-1.016 g/cm3

ε (extinction coefficient)

2.4 at 619 nm in H2O

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C
room temp

Quality Level

100

Related Categories

Analysis Note

1% solution in 3% acetic acid

Application

Alcianblue 8GX solution has been used as a staining dye for chondrocytic, osteoblastic and adipocytic differentiation. It has also been used for the staining of sections in immunohistochemistry.

General description

Alcian blue is a large conjugated dye molecule, and comprises of a central copper-containing pthalocyanine ring linked to four isothiouronium groups via thioether bonds. The isothiouronium groups are responsible for the cationic nature of alcian blue owing to them being moderately strong bases. It was initially used in dyeing of textile fibres. Alcian blue 8GX is a dye recommended normally for histological technique.

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN6211
BCCL9242
BCCK5618
BCCG7004
BCCC8957

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Oxygen tension regulates chondrocyte differentiation and function during endochondral ossification.
Hirao M et al.
The Journal of Biological Chemistry, 281, 31079-31079 (2006)
John D. Bancroft
Theory and Practice of Histological Techniques, 172-172 (2008)
Yong Mao et al.
Acta biomaterialia, 85, 75-83 (2018-12-12)
A significant expansion of autologous chondrocytes in vitro is required for cell-based cartilage repair. However, the in vitro expansion of chondrocytes under standard culture conditions inevitably leads to the dedifferentiation of chondrocytes and contributes to suboptimal clinical outcomes. To address
Aurora-a is essential for the tumorigenic capacity and chemoresistance of colorectal cancer stem cells.
Cammareri P et al.
Cancer Research, 70, 4655-4655 (2010)

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