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63064

Sigma-Aldrich

Magnesium chloride hexahydrate

BioUltra, ≥99.0% (KT)

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Synonym(s):
MgCl2.6H2O, dichloromangesium hexahydrate
Linear Formula:
MgCl2 · 6H2O
CAS Number:
7791-18-6
Molecular Weight:
203.30
EC Number:
232-094-6
MDL number:
MFCD00149781
UNSPSC Code:
12352302
PubChem Substance ID:
329759768
NACRES:
NA.26

product line

BioUltra

Quality Level

200

Assay

≥99.0% (KT)

form

solid

impurities

insoluble matter, passes filter test
≤0.0002% total nitrogen (N)

pH

5.0-7.5 (25 °C, 1 M in H2O)

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤50 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤2000 mg/kg
Li: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤3000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.030
λ: 280 nm Amax: 0.025

SMILES string

O.O.O.O.O.O.Cl[Mg]Cl

InChI

1S/2ClH.Mg.6H2O/h2*1H;;6*1H2/q;;+2;;;;;;/p-2

InChI key

DHRRIBDTHFBPNG-UHFFFAOYSA-L

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Application

  • Magnesium chloride hexahydrate has been used in wash buffer for the purification of protein from Saccharomyces cerevisiae.
  • It has been used for the preparation of extracellular (Ringer) solution for juxtacellular recording of single neurons in freely moving rats.
  • It has been used as a supplement to study effect of desiccation on bacterial viability.

Other Notes

Reconstitution of membrane processes; Strongly enhances the vinblastine-induced tubulin self-association; Component of Dulbecco′s phosphate buffered saline for tissue culture; Component of culture media for Pseudomonas. In Salmonella Enrichment Broth acc. to Rappaport, inhibits normal intestinal flora.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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In vitro Histone H3 Cleavage Assay for Yeast and Chicken Liver H3 Protease.
Chauhan S, et al.
Bio-protocol null
G D Eytan et al.
The Journal of biological chemistry, 251(21), 6831-6837 (1976-11-10)
Cytochrome oxidase, QH2-cytochrome c reductase, and the oligomycin-sensitive adenosine triphosphatase were incorporated into liposomes by a new procedure which yielded unidirectional orientation of the proteins. Cytochrome oxidase was reconstituted in the mitochrondrial orientation and the adenosine triphosphatase in the submitochondrial
Physical properties of purified calf brain tubulin.
G C Na et al.
Methods in enzymology, 85 Pt B, 393-408 (1982-01-01)
Effect of air drying on bacterial viability: A multiparameter viability assessment.
Nocker A, et al.
Journal of Microbiological Methods, 90, 86-86 (2012)
R DULBECCO et al.
The Journal of experimental medicine, 99(2), 167-182 (1954-02-01)
Plaques have been produced with the three types of poliomyelitis viruses on monolayer tissue cultures of monkey kidney and monkey testis. The number of plaques was proportional to the concentration of the virus. Each plaque originates, therefore, from a single
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