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61749

Millipore

Lauryl sulfate Broth

suitable for microbiology, NutriSelect® Basic

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Synonym(s):
Lauryl Casein peptone Broth
Beilstein:
1710530
MDL number:
MFCD00067711
UNSPSC Code:
41171607
PubChem Substance ID:
329759678
NACRES:
NA.74

Agency

according to GB 4789.3-2016
according to ISO 4831:2006
according to ISO

Quality Level

100

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

casein peptone, 20 g/L
dipotassium hydrogen phosphate, 2.75 g/L
lactose, 5 g/L
potassium dihydrogen phosphate, 2.75 g/L
sodium chloride, 5 g/L
sodium lauryl sulfate, 0.1 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

6.8±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring
microbiology

suitability

selective for Enterobacter spp.
selective for Escherichia coli
selective for Salmonella spp.
selective for coliforms

SMILES string

CCCCCCCCCCCCOS(O)(=O)=O

InChI

1S/C12H26O4S/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15/h2-12H2,1H3,(H,13,14,15)

InChI key

MOTZDAYCYVMXPC-UHFFFAOYSA-N

Related Categories

General description

Lauryl sulfate Broth (LSB) is a selective media, assortedly used for coliform determination. LSB serves to examine the quality of water, dairy products and foodstuffs.

Application

Lauryl sulfate Broth has been used for E.coli determination.
Selective medium acc. to Mallmann and Darby (1941) for the presumptive testing for coliform and their selective enrichment in the examination of water, dairy products and food.
Recommended by the "Schweizerisches Lebensmittelbuch" 5th ed., chapter 56A.
The difference to Cat. No. 17349 is that Casein Peptone is used (instead of Tryptose)

Preparation Note

Dissolve 35.6 g in 1 litre distilled water. Distribute to convenient flasks. Sterilize by autoclaving at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Uses of a Lauryl Sulfate Tryptose Broth for the Detection of Coliform Organisms.
W L Mallmann et al.
American journal of public health and the nation's health, 31(2), 127-134 (1941-02-01)
Determination of Escherichia coli in urine using a low-cost foil-based microfluidic device.
Maskova T, et al.
Talanta, 170, 36-40 (2017)

Articles

Differentiation and Detection of Escherichia coli, Coliforms and Enterobacteriaceae

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

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