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54232

Millipore

Lactose TTC Agar with Tergitol™-7

NutriSelect® Plus, suitable for microbiology

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Synonym(s):
TTC-Tergitol-7 Agar, m-Tergitol-7 Agar with Meat Extract
UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12.7 g/L
bromothymol blue, 0.05 g/L
lactose, 20.0 g/L
meat extract, 5 g/L
peptone, 10.0 g/L
tergitol-7, 0.1 g/L
yeast extract, 6.0 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

7.2±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring
microbiology

suitability

selective and differential for Citrobacter spp.
selective and differential for Enterobacter spp.
selective and differential for Escherichia coli
selective and differential for Proteus spp.
selective and differential for Salmonella spp.
selective and differential for Shigella spp.
selective and differential for coliforms

Related Categories

General description

Lactose TTC Agar with Tergitol™-7 is recommended as a selective and differential medium for the recovery of injured coliform organisms from chlorinated water by membrane filter technique.

Application

for detection and enumeration of coliform organisms from chlorinated water by membrane filter technique

Preparation Note

Suspend 53.85 g in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C. Aseptically add 2.5 ml of 1% 2,3,5 Triphenyl Tetrazolium chloride (TTC) solution (Cat. No. 17779).

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TERGITOL is a trademark of The Dow Chemical Company ("Dow") or an affiliated company of Dow

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Differentiation and Detection of Escherichia coli, Coliforms and Enterobacteriaceae

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

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