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Merck
CN

51558

HEPES buffered saline

BioUltra, Molecular Biology, 2× concentrate

Synonym(s):

HEPES Saline Buffer Solution

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About This Item

NACRES:
NA.25
PubChem Substance ID:
329757705
UNSPSC Code:
12161700
MDL number:
MFCD00006158

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Product Name

HEPES buffered saline, BioUltra, Molecular Biology, 2× concentrate

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

grade

Molecular Biology

product line

BioUltra

form

liquid

quality

2× concentrate

composition

dextrose, 2.0 g/L
HEPES, 10 g/L
KCl, 0.74 g/L
NaCl, 16 g/L
Na2HPO4.2H2O, 0.27 g/L

concentration

2 ×

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

refractive index

n20/D 1.339

pH

7.2 (25 °C)

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04

Quality Level

200

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Application

HEPES buffered saline has been used:
  • for the preparation of plasmid DNA
  • as a reagent to prepare transfection master mix for DNA construct vectors
  • to develop hydrogel precursor for cell leakage assay

General description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Preparation Note

concentration (in H2O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l dextrose

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH7391
BCCG6432
BCCD2118
BCBZ9638
BCBZ7440

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Labeling embryonic mouse central nervous system cells by in utero electroporation
Tabata H and Nakajima K
Development, Growth & Differentiation, 50(6), 507-511 (2008)
A new approach to neural cell culture for long-term studies
Potter SM and DeMarse TB
Journal of Neuroscience Methods, 110(1-2), 17-24 (2001)
Calcium phosphate transfection.
Kingston RE, Chen CA, Okayama H.
Current Protocols in Immunology, 10, 10-10 (2001)
Influence of HEPES buffer on the local pH and formation of surface layer during in vitro degradation tests of magnesium in DMEM
Dezfuli SN, et al.
Progress in Natural Science: Materials International, 24(5), 531-538 (2014)
Jong-Yuh Cherng et al.
Journal of materials science. Materials in medicine, 21(5), 1543-1551 (2010-02-06)
To improve the cytotoxicity of PEI25k and the transfection efficiency of poly(beta-amino ester) with DNA, we synthesized a poly(beta-amino ester), PEDP, bearing ester linkages in the backbone and tertiary amines in the backbone and side chain and prepared a binary
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