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4B200

Sigma-Aldrich

Sepharose™ 4B

45-165 μm bead diameter

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CAS Number:
9012-36-6
MDL number:
MFCD00081294
UNSPSC Code:
12352200
PubChem Substance ID:
24873317
NACRES:
NA.21

biological source

algae (marine)

Quality Level

200

form

suspension

technique(s)

affinity chromatography: suitable

bead diameter

45-165 μm

pore size

30,000-50,000 Da fractionation range (Dextrans)
60,000-20,000,000 Da fractionation range (Globular proteins)

storage temp.

2-8°C

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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General description

Sepharose™ 4B is an agarose bead and a polymer of Gal β1,4 [3,6]-anhydro-L-galactose. Sepharose aids in fractionating larger molecules and is devoid of charged saccharides. Presence of 3,6-anhydro-L-galactose resists biological degradation. Hydrogen bonds stabilize the sepharose gel structure. Sepharose is widely used in affinity chromatography by cross linking various ligands on the matrix.

Application

Sepharose™ 4B has been used:
  • to conjugate scorpion venom to be used in affinity chromatography
  • for clearing of protein extracts prior to immunoprecipitation
  • for gel purification and desalting of phospholipid vesicles
  • for adsorption of immunoglobulin G (IgG)

Legal Information

Sepharose is a trademark of Cytiva

replaced by

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

WGK

WGK 1

Flash Point(F)

100.4 - 109.4 °F

Flash Point(C)

38 - 43 °C

Regulatory Information

危险化学品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Construction of a liposome dialyzer for the preparation of high-value, small-volume liposome formulations
Adamala K, et al.
Nature Protocols, 10(6), 927-927 (2015)
Principles and Reactions of Protein Extraction, Purification, and Characterization (2004)
Oral Absorption of Mesobuthus eupeus Scorpion Venom in Mice.
Hosseini Z, et al.
Iranian Journal of Toxicology, 11(2), 15-19 (2017)
Paul Kaufmann et al.
PloS one, 14(8), e0220866-e0220866 (2019-08-08)
Dipeptidyl amino-peptidase 3 (DPP3) is an aminopeptidase involved in peptide degradation, including hormone peptides as angiotensin II and enkephalins. DPP3 plasma activity increases in septic patients and correlates with mortality risk. However, the exact physiological role of DPP3 remains unclear
Barna Dey et al.
Journal of virology, 81(11), 5579-5593 (2007-03-16)
The human immunodeficiency virus type 1 exterior gp120 envelope glycoprotein is highly flexible, and this flexibility may contribute to the inability of monomeric gp120 immunogens to elicit broadly neutralizing antibodies. We previously showed that an S375W modification of a critical
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