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Merck
CN

45347

Epoxy embedding medium, hardener MNA

≥95.0%

Synonym(s):

Methyl-5-norbornene-2,3-dicarboxylic anhydride, Epon™ MNA substitute, MNA, Methylnorbornene-2,3-dicarboxylic acid anhydride, Methyl nadic anhydride

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About This Item

Empirical Formula (Hill Notation):
C10H10O3
CAS Number:
25134-21-8
Molecular Weight:
178.18
NACRES:
MA.02
PubChem Substance ID:
24868813
UNSPSC Code:
41102904
EC Number:
246-644-8
Beilstein/REAXYS Number:
162395

Key Documents

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Product Name

Epoxy embedding medium, hardener MNA, ≥95.0%

InChI key

LTVUCOSIZFEASK-MPXCPUAZSA-N

InChI

1S/C10H10O3/c1-10-6-3-2-5(4-6)7(10)8(11)13-9(10)12/h2-3,5-7H,4H2,1H3/t5-,6+,7+,10+/m0/s1

SMILES string

[H][C@]12C3CC(C(C)=C3)[C@@]1([H])C(=O)OC2=O

biological source

synthetic

vapor density

6.1 (20 °C, vs air)

vapor pressure

5 mmHg ( 120 °C)

assay

≥95.0%

refractive index

n20/D 1.506 (lit.)
n20/D 1.507

density

1.232 g/mL at 25 °C (lit.)

application(s)

hematology
histology

storage temp.

room temp

Quality Level

100

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Application

It was used during transmission electron microscopy done for the analysis of epithelial cells; during a study performed to understand the epithelial cell growth and stratification in minimal culture conditions.

General description

Epoxy embedding medium is generally used for biological tissues during study by electron microscopy. Usually, it consists of three components; Epon 812 resin, an anhydride hardener and an accelerator.
additional information see 45359 Epoxy Embedding Medium kit

Legal Information

Epon is a trademark of Hexion, Inc.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Corr. 1C - Skin Sens. 1

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 1

flash_point_f

275.0 °F

flash_point_c

135 °C

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN9356
BCCL9788
BCCK0626
BCCJ0027
BCCF9043

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SECTION STAINING FOR ELECTRON MICROSCOPY Incompatibility of Methyl Nadic Anhydride with Permanganates.
Reedy, Michael K.
The Journal of Cell Biology, 26.1, 309-311 (1965)
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Federica Riva et al.
Methods in molecular biology (Clifton, N.J.), 585, 25-43 (2009-11-13)
Biological risk management is required in modern tissue engineering. Particular attention should be paid to the culture medium and the scaffold used. In this perspective, it is important to define minimal culture conditions which allow proper growth and differentiation of
Xiao-Wei Zhang et al.
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Impaired macroautophagy/autophagy is involved in the pathogenesis of hepatic fibrosis. However, how aberrant autophagy promotes fibrosis is far from understood. Here, we aimed to define a previously unrevealed pro-fibrotic mechanism for the stress protein TRIB3 (tribbles pseudokinase 3)-mediated autophagy dysfunction.
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