43925
Nigrosin Stain solution
suitable for microscopy
form
liquid
shelf life
limited shelf life, expiry date on the label
technique(s)
microbe id | staining: suitable
suitability
suitable for microscopy, Bacillus spp., Corynebacterium spp.
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
General description
Negative staining method permits visualization of the usually transparent and unstainable capsule of many organisms, most importantly Cryptococcus neoformans.
Other Notes
Composition:
Nigrosin 100 g/l, Formalin 5 ml/l in water
Nigrosin 100 g/l, Formalin 5 ml/l in water
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B - Skin Sens. 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves
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Leonardo F C Brito et al.
Theriogenology, 60(8), 1539-1551 (2003-10-02)
The objectives of this study were to compare different methods of evaluating sperm plasmalemma and to determine their relationship with in vitro fertilization rate. A single batch of frozen semen from each of eight beef bulls was used for assessment
Jesse Schallek et al.
Investigative ophthalmology & visual science, 52(3), 1325-1335 (2010-11-06)
To examine the extent to which neurovascular coupling contributes to stimulus-evoked intrinsic signals in the retina. The retinas of five adult cats were examined in vivo. Animals were anesthetized and paralyzed for imaging stability. The retinas were imaged through a
K Matsuda et al.
Journal of clinical laboratory analysis, 15(4), 171-174 (2001-07-04)
We designed a semiquantitative analysis of urinary low protein levels using silver dot blot assay. In this method, 3 microl of urine are blotted to one dot onto a cellulose acetate membrane, which is stained by a colloidal silver staining
M L Foster et al.
Theriogenology, 76(2), 334-341 (2011-04-19)
Sperm membrane integrity (SMI) is thought to be an important measure of stallion sperm quality. The objective was to compare three methods for evaluating SMI: flow cytometry using SYBR-14/propidium iodide (PI) stain; an automated cell counting device using PI stain;
Shuai Zhang et al.
Journal of colloid and interface science, 316(1), 168-174 (2007-08-31)
A convenient approach was developed to fabricate monodisperse nigrosine-doped poly(methyl methacrylate-co-divinylbenzene-co-methacrylic acid) nanoparticles with different cross-linkage by soap-free emulsion polymerization at boiling status and swelling process. The dye-doped nanoparticles were used for the fabrication of colloidal crystal films and beads.
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