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43052

Millipore

Müller-Kauffmann Tetrathionate Broth, Base (ISO)

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Tetrathionate Müller-Kaufmann Broth Base

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.85

Agency

according to ISO 19250:2010
according to ISO 6579-1:2017

Quality Level

200

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

bile salts, 4.78 g/L
calcium carbonate, 38.7 g/L
casein peptone, 8.60 g/L
meat extract, 4.30 g/L
sodium chloride, 2.60 g/L
sodium thiosulfate, 47.80 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

application(s)

clinical testing
environmental
food and beverages
microbiology

suitability

selective for Salmonella spp.
selective for Shigella spp.

General description

Müller-Kauffmann Tetrathionate Broth, Base (ISO) is a selective enrichment of Salmonellae from various sources like milk and other food items.

Preparation Note

Suspend 107 g of powder in 1 litre of distilled water, mix well and bring to the boil and let it cool to 40-45°C. Immediately before use add 20 ml of iodine-iodide solution prepared by dissolving 5 g of potassium iodide in 10 ml of water, adding 6 g of iodine and then diluting to 20 ml with sterile water. Also add the contents of two vials of Brilliant Green Novobiocin Selective Supplement (Cat. No. 55181) reconstituted as directed. Mix well and aseptically dispense into sterile tubes. Do not heat after adding the iodine solution. Complete medium must be used immediately; the base, without iodine, may be stored in the refrigerator for some days. White precipitate is due to calcium carbonate and is must be considered as normal.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

新产品

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Certificates of Analysis (COA)

Lot/Batch Number
BCCC6335
BCBR6345V

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Differentiation and ID Media for Salmonella

Salmonella, with 2,300+ serotypes, causes half of food-borne illnesses, often from dairy, poultry, and eggs.

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