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39894

Millipore

EC O157 ChromoSelect Agar

NutriSelect® Plus, suitable for microbiology

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Synonym(s):
EC O157 Agar ChromoSelect
MDL number:
MFCD03457068
UNSPSC Code:
41106200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12.0 g/L
bile salts mixture, 1.5 g/L
casein enzymic hydrolysate, 8.0 g/L
chromogenic mixture, 0.25 g/L
sodium lauryl sulfate, 0.1 g/L
sorbitol, 7.0 g/L

manufacturer/tradename

NutriSelect® Plus

application(s)

agriculture
clinical testing
environmental
food and beverages
microbiology

storage temp.

2-8°C

suitability

selective and differential for Escherichia coli
selective and differential for coliforms
selective and differential for enterovirulent E. coli (EEC)

Application

EC O157 ChromoSelect Agar is a chromogenic medium for isolation and differentiation of E. coli O157 from food and environmental samples.

Preparation Note

Suspend 28.85 g in 1 liter distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50°C and pour into sterile petri plates. This medium can be made more selective by aseptically adding 0.25 ml of 1% potassium tellurite solution (Cat. No. 17774) to 1 liter molten and cooled medium (45°C).

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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P M Zadik et al.
Journal of medical microbiology, 39(2), 155-158 (1993-08-01)
Potassium tellurite was assessed for the selection of verocytotoxigenic (VT+) Escherichia coli O157. MICs were higher for VT+ E. coli O157 than for other strains of E. coli and for Aeromonas spp. MacConkey medium containing sorbitol, tellurite and cefixime (TC-SMAC)
Media for the isolation and differentiation of pathogenic Esch. coli (serotypes O 111 and O 55).
F RAPPAPORT et al.
Journal of clinical pathology, 5(4), 361-362 (1952-11-01)

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