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39727

Millipore

Perfringens Agar Base

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSC Code:
41171609
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

composition

Agar, 15 g/L
Beef extract, 5 g/L
Ferric ammonium citrate, 1 g/L
Papaic digest of soybean meal, 5 g/L
Sodium metabisulphite, 1 g/L
Tryptose, 15 g/L
Yeast extract, 5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

application(s)

bioburden testing
cleaning products
cosmetics
environmental
food and beverages
personal care
microbiology

suitability

selective and differential for Clostridium spp.

Related Categories

General description

Perfringens Agar Base with the addition of selective supplement and enrichment is used for the presumptive identification and enumeration of Clostridium perfringens from food.

Application

Perfringens Agar Base has been used in the cell culture of Clostridium perfringens.

Preparation Note

Suspend 23.5 g of Perfringens Agar Base in 475 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C. Add 25 ml of Egg Yolk Emulsion (Cat. No. 17148) and additionally the rehydrated contents of 1 vial of Perfringens S.F.P. Supplement (Cat. No. 53436) or 1 vial of Perfringens T.S.C. Supplement (Cat. No. P9352). Mix well and pour into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Martina Hustá et al.
Anaerobe, 63, 102205-102205 (2020-05-01)
Isolation and fast detection of Clostridium perfringens is essential in veterinary medical diagnostics and veterinary research, as it allows to recommend suitable treatment options after antimicrobial resistance determination, and is essential to study pathogenesis. In this study four selective media

Articles

Detection, Identification and Differentiation of Clostridium perfringens

Clostridium perfringens can cause contamination in undercooked or improperly sterilized canned foods and water. Learn to detect, identify, and differentiate this pathogen.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service