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Merck
CN

39441

Nitrate Reagent B

suitable for microbiology

Synonym(s):

Sulfanilic acid solution, 4-Aminobenzenesulfonic acid

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About This Item

CAS Number:
NACRES:
NA.85
PubChem Substance ID:
UNSPSC Code:
41171621
MDL number:
Technical Service
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shelf life

limited shelf life, expiry date on the label

Quality Level

composition

acetic acid (30%), 1000 mL , sulfanilic acid, 8.0 g

technique(s)

microbe id | metabolite detection: suitable

application(s)

agriculture
clinical testing
environmental
food and beverages
pharmaceutical, microbiology

suitability

Enterobacter spp., Escherichia coli, Neisseria spp., anaerobic bacteria, coliforms

SMILES string

Nc1ccc(cc1)S(O)(=O)=O

InChI

1S/C6H7NO3S/c7-5-1-3-6(4-2-5)11(8,9)10/h1-4H,7H2,(H,8,9,10)

InChI key

HVBSAKJJOYLTQU-UHFFFAOYSA-N

Application

α-Naphthylamine and sulfanilic acid are used for detection of nitrate reduction by bacteria. Organisms containing nitrate reductase reduce nitrate to nitrite, which forms a diazonium salt with sulfanilic acid and reacts with α-naphthylamine to form a red azo dye.


pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Regulatory Information

危险化学品

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Articles

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram.

There are many other methods of detection to indicate the presence of E. coli. Review common tests and biochemical reactions for this contaminant.

Specific agars and broths support detection and cultivation of Campylobacter, requiring complex media with essential supplements.

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Related Content


Kwan Kim et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 100, 10-14 (2012-02-14)
We estimated the apparent size of the 'hot site' for surface-enhanced Raman scattering (SERS) located within the gaps between Pt nanoparticles and a flat Ag substrate. Initially, no Raman peaks were detected for 4-aminobenzenethiol (4-ABT) on a flat Ag substrate.
Han Ming Gan et al.
Chemosphere, 82(4), 507-513 (2010-11-26)
A co-culture consisting of Hydrogenophaga sp. PBC and Ralstonia sp. PBA, isolated from textile wastewater treatment plant could tolerate up to 100 mM 4-aminobenzenesulfonate (4-ABS) and utilize it as sole carbon, nitrogen and sulfur source under aerobic condition. The biodegradation
Han Ming Gan et al.
Microbiology (Reading, England), 158(Pt 8), 1933-1941 (2012-05-23)
The gene coding for the oxygenase component, sadA, of 4-aminobenzenesulfonate (4-ABS) 3,4-dioxygenase in Hydrogenophaga sp. PBC was previously identified via transposon mutagenesis. Expression of wild-type sadA in trans restored the ability of the sadA mutant to grow on 4-ABS. The



Global Trade Item Number

SKUGTIN
39441-100ML-F04061831982336