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Merck
CN

365548

Direct Red 80

Dye content 25 %

Synonym(s):

Sirius Red

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About This Item

Empirical Formula (Hill Notation):
C45H26N10Na6O21S6
CAS Number:
2610-10-8
Molecular Weight:
1373.07
EC Number:
220-027-3
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24862536
MDL number:
MFCD00054389
Colour Index Number:
35780
Beilstein/REAXYS Number:
8185575

Key Documents

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Product Name

Direct Red 80, Dye content 25 %

InChI key

YIQKLZYTHXTDDT-IJPLDVMMSA-H

InChI

1S/C45H32N10O21S6.6Na/c56-43-33-13-5-27(17-23(33)19-39(81(71,72)73)41(43)54-52-35-15-7-29(21-37(35)79(65,66)67)50-48-25-1-9-31(10-2-25)77(59,60)61)46-45(58)47-28-6-14-34-24(18-28)20-40(82(74,75)76)42(44(34)57)55-53-36-16-8-30(22-38(36)80(68,69)70)51-49-26-3-11-32(12-4-26)78(62,63)64;;;;;;/h1-22,56-57H,(H2,46,47,58)(H,59,60,61)(H,62,63,64)(H,65,66,67)(H,68,69,70)(H,71,72,73)(H,74,75,76);;;;;;/q;6*+1/p-6/b50-48+,51-49+,54-52+,55-53+;;;;;;

SMILES string

[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c(cc4cc(NC(=O)Nc5ccc6c(O)c(\N=N\c7ccc(cc7S([O-])(=O)=O)\N=N\c8ccc(cc8)S([O-])(=O)=O)c(cc6c5)S([O-])(=O)=O)ccc14)S([O-])(=O)=O

form

powder

composition

Dye content, 25%

technique(s)

titration: suitable

color

dark brown

λmax

528 nm

ε (extinction coefficient)

≥12000 at 277-283 nm in H2O
≥15000 at 223-235 nm in H2O
≥32000 at 524-530 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Direct Red 80 has been used as an ingredient in the preparation of saturated picric acid for immunohistochemical detection and quantitation of collagen in liver tissues.Other applications include -
  • detection of amyloid
  • Sirius red-Van Gieson methods for quantitation of collagen using image analysis methods
  • counterstaining applications substituting eosin in alum hematoxylins

General description

Direct Red 80 (C.I. 35780) is an extremely hydrophilic, very large tetrakisazo hexasulfonated anionic dye that has a large, planar aromatic system.It is also known as Sirius red F3B, chlorantine fast red 7BLN or 5BRL, durazol brilliant red B, pontamine fast red 7BNL, and sirius fast red.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000517177
0000517600
0000517600
0000517177
0000488094

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George J, et al.
Proceedings of the National Academy of Sciences of the USA, 96(22), 12719-12724 (1999)
Sabatini M, et al.
Cartilage and Osteoarthritis (2004)
Degradation of a Textile Dye C. I. Direct Red 80 by
Electrochemical Processes
.
Lopes A, et al.
Portugaliae Electrochimica Acta , 279-294 (2004)
Christopher A Miller et al.
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Extracellular matrix expansion is a key element of ventricular remodeling and a potential therapeutic target. Cardiovascular magnetic resonance (CMR) T1-mapping techniques are increasingly used to evaluate myocardial extracellular volume (ECV); however, the most widely applied methods are without histological validation.
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