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Fehling′s reagent I for sugars

reag. Ph. Eur., for determination of sugar, solution I: copper(II) sulfate

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Synonym(s):
Copper(II) sulfate solution, Cupric sulfate solution acc. to Fehling, Cupric sulfate standard
CAS Number:
7758-99-8
MDL number:
MFCD00010981
UNSPSC Code:
12164500
PubChem Substance ID:
329755253
NACRES:
NA.21

grade

for determination of sugar

Quality Level

100

Agency

USP/NF
reag. Ph. Eur.

concentration

17.6 g/L±0.1 g/L Cu

density

1.038-1.060 g/mL at 20 °C

suitability

in accordance for application

application(s)

food and beverages
general analytical
pharmaceutical (small molecule)

SMILES string

[Cu++].[O-]S([O-])(=O)=O

InChI

1S/Cu.H2O4S/c;1-5(2,3)4/h;(H2,1,2,3,4)/q+2;/p-2

InChI key

ARUVKPQLZAKDPS-UHFFFAOYSA-L

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General description

The principle behind Fehling′ test is basically based on the reducing property of monosaccharide and disaccharides, which in turn depends on the presence of free keto or an aldehyde group. This test is performed by adding a few drops of sugar solution to a mixture of Fehling′s A solution (i.e., CuSO4) and Fehling′s B solution (i.e., KOH and Na-K tartarate). Fehling′s solution gets reduced by aldose sugars to insoluble yellow or red colored cuprous oxide. Monosaccharides like glucose, galactose and fructose and disaccharides like lactose and maltose resond freely to Fehling′s test as they possess a free keto or an aldehyde group, whereas sucrose fails to answer the test as it does not possess a free aldehyde or an keto group. Hence, Fehling′s test is also used to distinguish reducing discaccharides like maltose from non-reducing sugars like sucrose.

Pictograms

Environment
GHS09

Signal Word

Warning

Hazard Statements

H410

Precautionary Statements

P273 - P391 - P501

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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