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34854-M

Sigma-Aldrich

Chloroform

suitable for HPLC, ≥99.8%, amylene stabilized

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Synonym(s):
Methylidyne trichloride, Trichloromethane
Empirical Formula (Hill Notation):
CHCl3
CAS Number:
67-66-3
Molecular Weight:
119.38
Beilstein:
1731042
EC Number:
200-663-8
MDL number:
MFCD00000826
UNSPSC Code:
12190000
PubChem Substance ID:
329755063
NACRES:
NA.21

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

Assay

≥99.8%

form

liquid

quality

amylene stabilized

technique(s)

HPLC: suitable

impurities

Free acid, in accordance
 acetone, aldehyde, in accordance (nessler test)
 free chlorine (Cl), in accordance
≤0.0005% non-volatile matter
≤0.001% free acid (as HCl)
<0.01% water (Karl Fischer)

evapn. residue

≤0.0005%

color

APHA: ≤10

transmittance

250 nm, ≥50%
260 nm, ≥85%
280 nm, ≥99%

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.48 g/mL at 25 °C
1.492 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): in accordance

cation traces

Pb: ≤0.05 mg/kg

absorption

in accordance

λ

H2O reference

UV absorption

λ: 250 nm Amax: ≤1.00
λ: 260 nm Amax: ≤0.30
λ: 280 nm Amax: ≤0.005

suitability

passes test for reaction against H2SO4
passes test for suitability of determ. w. dithizone

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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General description

Chloroform, a halogenated hydrocarbon, is widely employed as a solvent. Its photochemical degradation has been investigated in the presence of TiO2 aqueous suspensions (wavelength range of 310-380nm).

Application

Chloroform has been employed for the ion exchange isolation of sulfatides from the urinary samples by using DEAE-cellulose membranes before the tandem mass spectrometric analysis. It has been used as a solvent for the Langmuir-Blodgett (LB) deposition of lipid bilayers over mica sheets (silver coated back).†

Other Notes

Important notice
  • The article number 34854-4X2.5L-M will be discontinued. Please order the single bottle 34854-2.5L-M which is physically identical with the same exact specifications.
  • The article number 34854-6X1L-M will be discontinued. Please order the single bottle 34854-1L-M which is physically identical with the same exact specifications.

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Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Target Organs

Central nervous system

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Ladislav Kuchař et al.
Clinica chimica acta; international journal of clinical chemistry, 425, 153-159 (2013-07-11)
Prediagnostic steps in suspected metachromatic leukodystrophy (MLD) rely on clinical chemical methods other than enzyme assays. We report a new diagnostic method which evaluates changes in the spectrum of molecular types of sulfatides (3-O-sulfogalactosyl ceramides) in MLD urine. The procedure

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