All Photos(2)
Synonym(s):
1-(2-Nitrobenzylidenamino)-2,4-imidazolidinedione
Empirical Formula (Hill Notation):
C10H8N4O4
CAS Number:
Molecular Weight:
248.19
MDL number:
UNSPSC Code:
41116107
NACRES:
NA.24
Recommended Products
grade
analytical standard
Quality Level
product line
VETRANAL®
shelf life
limited shelf life, expiry date on the label
technique(s)
HPLC: suitable
gas chromatography (GC): suitable
application(s)
clinical testing
format
neat
SMILES string
O=C(C1)NC(N1/N=C/C2=C([N+]([O-])=O)C=CC=C2)=O
InChI
1S/C10H8N4O4/c15-9-6-13(10(16)12-9)11-5-7-3-1-2-4-8(7)14(17)18/h1-5H,6H2,(H,12,15,16)/b11-5+
InChI key
FULJCJZKZXOFQZ-VZUCSPMQSA-N
General description
2-NP-AHD belongs to the class of nitrofurans (NFs), a series of synthetic broad spectrum antibiotics, used for veterinary applications.
2-NP-AHD is a metabolite of nitrofurans type of antibiotics generally used as veterinary drugs.
Application
2-NP-AHD may be used as an analytical reference standard for the determination of the analyte in shrimp and aquatic products using ultra performance liquid chromatography(UPLC) and liquid chromatography(LC) coupled to tandem mass spectrometry (MS/MS).
2-NP-AHD may be used as an analytical standard for the determination of 2-NP-AHD in:
- Honey by modified QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction followed by liquid chromatography-tandem spectrometry (LC-MS/MS) with multiple reaction monitoring (MRM) detection and visualized microarray screen assay.
- Animal-derived foods by water-compatible molecularly imprinted solid-phase extraction (SPE) and high performance LC with MS/MS.
- Katla fish (Katla katla) and Bagda shrimp (Penious monodon) by ultra-performance LC-MS/MS.
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Other Notes
Derivatized standard for the assay of nitrofurans in animal tissue by LC/MS
Recommended products
Find a digital Reference Material for this product available on our online platform ChemisTwin® for NMR. You can use this digital equivalent on ChemisTwin® for your sample identity confirmation and compound quantification (with digital external standard). An NMR spectrum of this substance can be viewed and an online comparison against your sample can be performed with a few mouseclicks. Learn more here and start your free trial.
Legal Information
VETRANAL is a registered trademark of Merck KGaA, Darmstadt, Germany
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
A SIMULTANEOUS METHOD DEVELOPMENT, VALIDATION AND CONFIRMATORY ANALYSIS OF AMOZ, AOZ, AHD AND SEM IN KATLA FISH (KATLA KATLA) AND BAGDA SHRIMP (PENIOUS MONODON) BY UPLC-MS/MS (QPXE).
Alam MA and Muhammad T
Journal of Biotechnology, 2(3), 102-112 (2014)
Development of water-compatible molecularly imprinted solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry for the detection of six sulfonamides in animal-derived foods.
Zhao X, et al.
Journal of Chromatography A, 1574, 9-17 (2018)
Determination of Nitrofuran Metabolites in Shrimp by LC/MS/MS.
Ye L, et al.
Laboratory Information Bulletin, 4466, 1-20 (2011)
Simultaneous detection of four nitrofuran metabolites in honey by using a visualized microarray screen assay.
Zhoumin L, et al.
Food Chemistry, 190, 982-989 (2017)
Development and validation of a modified QuEChERS protocol coupled to LC-MS/MS for simultaneous determination of multi-class antibiotic residues in honey.
Shendy AH, et al.
Food Chemistry, 190, 982-989 (2016)
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