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Supelco

1,5-Diphenylcarbazide

reag. Ph. Eur., ≥98.0%, for metal titration

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Synonym(s):
1,5-Diphenylcarbohydrazide, sym.-Diphenylcarbazide
Linear Formula:
C6H5NHNHCONHNHC6H5
CAS Number:
140-22-7
Molecular Weight:
242.28
Beilstein:
752039
EC Number:
205-403-7
MDL number:
MFCD00003013
UNSPSC Code:
41116105
PubChem Substance ID:
329754464
NACRES:
NA.21

grade

for metal titration

Quality Level

200

Agency

USP/NF
reag. Ph. Eur.

Assay

≥98% (HPLC)
≥98.0%

technique(s)

UV/Vis spectroscopy: suitable
titration: suitable

impurities

diphenylcarbazone, in accordance
≤0.1% insoluble matter in ethanol

ign. residue

≤0.05% (as SO4)

mp

170-175 °C (lit.)
170-175 °C

suitability

passes test for Hg detection

SMILES string

O=C(NNc1ccccc1)NNc2ccccc2

InChI

1S/C13H14N4O/c18-13(16-14-11-7-3-1-4-8-11)17-15-12-9-5-2-6-10-12/h1-10,14-15H,(H2,16,17,18)

InChI key

KSPIHGBHKVISFI-UHFFFAOYSA-N

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General description

1,5-Diphenylcarbazide reaction with chromium(VI), has been widely used for the spectro- photometric determination of chromium. It is also an electron donor, and can be used for measuring photosystem 2 activity in subchloroplast fragments.

Application

1,5-diphenylcarbazide was used for preparing diphenylcarbazide solution, during an experimental procedure done for reducing biological chromium(VI), using trickling filter. It may be used for pre-column complexation in HPLC, during on-line preconcentration and determination of chromium(VI) traces in water.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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On-line preconcentration and determination of chromium (VI) in waters by high-performance liquid chromatography using pre-column complexation with 1, 5-diphenylcarbazide.
Padarauskas A
Journal of Chromatography A, 808 (1), 193-199 (1998)
Biological chromium (VI) reduction using a trickling filter.
Dermou E
Journal of Hazardous Materials, 126 (1), 78-85 (2005)
Oxidation of 1, 5-diphenylcarbazide as a measure of photosystem 2 activity in subchloroplast fragments.
Vernon LP and Shaw ER.
Biochemical and Biophysical Research Communications, 36 (6), 878-884 (1969)
W Hillier et al.
Biochemistry, 36(1), 76-85 (1997-01-07)
The behavior of photosystem II (PSII) reactions was investigated under conditions of decreasing water content by the addition of increasing concentrations of ethylene glycol (EG). The photosynthetic activities were measured for PSII samples either directly in aqueous solutions of EG
G Samanta et al.
Analytical chemistry, 73(9), 2034-2040 (2001-05-17)
An automated continuous instrument for the collection and measurement of aerosol Cr(VI) is described. The system alternately collects the sample on one of two glass fiber filters. After 15-min sample collection on one filter, the sampling switches over to the
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