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Merck
CN

32750

3,3′-Diaminobenzidine tetrahydrochloride hydrate

for spectrophotometric det. of Se, ≥97.5%

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About This Item

Linear Formula:
C12H14N4 · 4HCl · xH2O
CAS Number:
868272-85-9
NACRES:
NA.21
PubChem Substance ID:
329754314
UNSPSC Code:
41116105
EC Number:
231-018-9
MDL number:
MFCD08273058

Key Documents

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Product Name

3,3′-Diaminobenzidine tetrahydrochloride hydrate, for spectrophotometric det. of Se, ≥97.5%

InChI key

DXWSCXIZIHILNP-UHFFFAOYSA-N

InChI

1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2

SMILES string

O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

assay

≥97.5%

quality

for spectrophotometric det. of Se

technique(s)

UV/Vis spectroscopy: suitable

Quality Level

100

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Application

3,3′-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex.

General description

3,3′-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) is toxic in nature, and is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels.

Other Notes

Reagent for the spectrophotometric determination of selenium; Peroxidase stain. Differentiation between two classes of mycobacterial catalase in polyacrylamide electrophoresis gels; Specific indication of hemoproteins in polyacrylamide gels by double staining

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

危险化学品
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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ1210
MKCX2929
MKCV5872
MKCW7760
MKCK5098

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HistoGreen: a new alternative to 3, 3'-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) as a peroxidase substrate in immunohistochemistry"
Thomas MA and Lemmer B
Brain Research, 14 (2), 107-118 (2005)
L G Wayne et al.
Analytical biochemistry, 157(1), 89-92 (1986-08-15)
Mycobacteria produce two classes of catalase, designated T and M. Only the T-catalase also has a peroxidase-like function. When a 3,3'-diaminobenzidine (DAB) peroxidase stain was applied to polyacrylamide gel electrophoresis gels, followed by a ferricyanide negative stain for catalase, isoenzymes
Danzuka, T. and Ueno, K.
Analytical Chemistry, 30, 1370-1370 (1958)
Interleukin-8 in the Human Endometrium 1.
Ariciri A
J. Clin. Endocr., 83 (5), 1783-1787 (1998)
R T Francis et al.
Analytical biochemistry, 136(2), 509-514 (1984-02-01)
Hemoproteins were revealed in polyacrylamide gels in the presence of sodium dodecyl sulfate by staining with different benzidine derivatives. When the protein samples were treated with either beta-mercaptoethanol or dithiothreitol, a significant decrease in peroxidase activity of the proteins possessing
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