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Merck
CN

318752

Bromothymol Blue sodium salt solution

0.04 wt. % in H2O

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About This Item

Empirical Formula (Hill Notation):
C27H27Br2NaO5S
CAS Number:
34722-90-2
Molecular Weight:
646.36
NACRES:
NA.47
PubChem Substance ID:
24859144
UNSPSC Code:
12171500
MDL number:
MFCD00077263

Key Documents

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Product Name

Bromothymol Blue sodium salt solution, 0.04 wt. % in H2O

InChI

1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;

SMILES string

[Na+].CC(C)c1cc(c(C)c(Br)c1O)\C(=C2/C=C(C(C)C)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O

InChI key

NMKFVGALBGZKGW-FKWCIMQXSA-M

form

liquid

concentration

0.04 wt. % in H2O

pH

6.0-7.6, yellow to blue

density

0.99 g/mL at 25 °C (lit.)

λmax

615 nm

ε (extinction coefficient)

≥13000 at 304-310 nm in 0.1 M NaOH at 0.005 g/L
≥8000 at 391-397 nm in 0.1 M NaOH at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

Related Categories

Application

Bromothymol blue has various applications in biology and medicine. It is suitable for demonstrating fungal hyphae within plant roots. It is used as a vital stain to trace the movement of fluids from the lymph. It is added to an agar gel microbial culture medium for enumeration of Bacillus cereus. Bromothymol blue is also used as a pH indicator due to its precise color transition properties at neutrality.

Biochem/physiol Actions

Bromothymol Blue is a pH indicator which changes color from yellow (pH 6.0) to blue (7.6). It belongs to the class of sulfonephthalein dyes.

General description

Bromothymol Blue is a sulfonated hydroxyquinone in acidic solutions. In alkaline solutions, it is present as a dianion that is blue with a larger conjugated system than the yellow hydroxyquinone. Both forms are lipophilic. It is also known as bromthymol blue or dibromothymolsulfonphthalein.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBR7203
MKCK5983
MKCG1338
MKBW6256V
S66648V

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R. W. Sabnis
Handbook of Acid-Base Indicators (2007)

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