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30787

Millipore

Deoxyribonuclease Test Agar

NutriSelect® Plus, suitable for microbiology

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

100

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
deoxyribonucleic acid, 2 g/L
sodium chloride, 5 g/L
tryptose, 20 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.3±0.2 (37 °C)

application(s)

clinical testing
environmental
food and beverages
microbiology

suitability

nonselective and differential for Serratia spp.
nonselective and differential for Staphylococcus spp.
nonselective and differential for Streptococcus spp.

General description

Deoxyribonuclease Test Agar is recommended for the detection of deoxyribonuclease activity of bacteria and fungi, and especially for identification of pathogenic Staphylococci.
Bacteria are streaked on to the surface of the agar medium and incubated. The growth on the surface of the agar is then flooded with 1N hydrochloric acid. Polymerised DNA precipitates in the presence of 1N HCl and makes the medium opaque. If the organisms produce DNase enzymes, in sufficient quantity to hydrolyse the DNA, then clear zones are seen around the colonies.

Application

for the detection of deoxyribonuclease activity of bacteria and fungi, and especially for identification of pathogenic Staphylococci

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCBR7992V
BCBR5479V

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M M STREITFELD et al.
Journal of bacteriology, 84, 77-80 (1962-07-01)
Streitfeld, Murray M. (University of Miami, Coral Gables, Fla.), Edward M. Hoffmann, and Herbert M. Janklow. Evaluation of extracellular deoxyribonuclease activity in Pseudomonas. J. Bacteriol. 84:77-80. 1962.-Of 23 strains of P. aeruginosa isolated from pathological processes, 22 strains, representative of
Rapid method for determining the activity of microorganisms on nucleic acids.
C D JEFFRIES et al.
Journal of bacteriology, 73(4), 590-591 (1957-04-01)
Modification of deoxyribonuclease test medium for rapid identification of Serratia marcescens.
J B Schreier
American journal of clinical pathology, 51(6), 711-716 (1969-06-01)
Deoxyribonuclease activity of micrococci from clinical sources.
B G WECKMAN et al.
Journal of bacteriology, 73(6), 747-753 (1957-06-01)

Articles

Media for Staphylococcus Aureus Detection

Chromogenic media enable the selective detection of S. aureus, which produce bluish-green colonies that are clearly differentiated from other species.

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