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Sigma-Aldrich

Methylene Blue hydrate

≥95% (calc. to the dried substance)

Synonym(s):

Basic Blue 9 monohydrate ; Methylene Blue (hydrate)

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About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S · xH2O
CAS Number:
122965-43-9
Molecular Weight:
319.85 (anhydrous basis)
Beilstein:
3599847
EC Number:
200-515-2
MDL number:
MFCD00150006
UNSPSC Code:
12171500
PubChem Substance ID:
329753217
NACRES:
MA.02

Quality Level

200

Assay

≥95% (calc. to the dried substance)

form

powder

impurities

≤1% insoluble in ethanol

loss

≤22% loss on drying, 105 °C

color

very dark green

solubility

ethanol: insoluble ≤1%

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1

InChI key

WQVSELLRAGBDLX-UHFFFAOYSA-M

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General description

Methylene blue is bis(dimethylamino)phenothiazine hydrochloride (tetramethylthionine),
C16H18N3SCl. It is a major basic histological dye in pharmacology and microbiology. This heterocyclic aromatic chemical appears as a dark green solid powder, changing to blue in an aqueous solution. Methylene blue is tested for histological and and bacteriological staining, and as a constituent of Wright’s blood stain.

Application

Methylene blue is widely used in several routine staining applications including Mallory’s phloxine-methylene blue on paraffin sections of tissue fixed in Zenker’s fluid for nuclear staining and bacterial staining in milk. It is also suitable for manufacturing the polychrome methylene blue used in the preparation of Romanowsky stains.Methylene Blue hydrate has also been used:
  • in the preparation of methylene blue (MB) solution
  • to stain zymograms -to label the injection site in the tissue slices
  • to pre-treat mice via oral gavage to study its effects on noise-induced hearing loss

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302

Precautionary Statements

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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