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27048

Millipore

Christensen′s Urea Agar

suitable for microbiology, NutriSelect® Basic

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Synonym(s):
Urea Agar Base acc. to Christensen, Urea Test Agar
UNSPSC Code:
41171606
NACRES:
NA.74

Agency

according to GB 4789.4-2016
according to ISO 10273:2017
according to ISO 19250:2010
according to ISO 6579-1:2017

Quality Level

200

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12 g/L
gelatine peptone, 1 g/L
D(+)-glucose, 1 g/L
phenol red, 0.012 g/L
potassium dihydrogen phosphate, 2 g/L
sodium chloride, 5 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

final pH

6.8±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
microbiology

suitability

nonselective and differential for Enterobacter spp.
nonselective and differential for Escherichia coli
nonselective and differential for Klebsiella spp.
nonselective and differential for Proteus spp.
nonselective and differential for Yersinia spp.
nonselective and differential for coliforms

Related Categories

Application

For the differentiation of urea-metabolizing microorganisms. Weak urease producers such as klebsiella, enterobacter etc. can also be differentiated. Yersinia pseudotuberculosis (urea positive) from Yersinia pestis (urea negative) acc. to Thal and Chen (1955).

Preparation Note

Dissolve 21 g in 950 ml distilled water. Sterilize by autoclaving at 121°C for 15 minutes. After cooling to 50°C, add aseptically 50ml (10 vials) sterile filtered 40% urea solution (Cat. No. 08582) and let cool as slants.

Other Notes

Screening of enteric pathogens from stool specimens

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物来源培养基

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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W.B. Christensen
Journal of Bacteriology, 69, 103-103 (1946)
Two simple tests for the differentiation of plague and pseudo tuberculosis bacilli.
E THAL et al.
Journal of bacteriology, 69(1), 103-104 (1955-01-01)
P A Villasante et al.
Journal of clinical microbiology, 25(3), 584-585 (1987-03-01)
A total of 800 colonies suggestive of Salmonella, Shigella, or Yersinia species isolated on stool differential agar media were inoculated onto both conventional biochemical test media (triple sugar iron agar, urea agar, and phenylalanine agar) and Entero Pathogen Screen cards

Articles

Differentiation and Detection of Escherichia coli, Coliforms and Enterobacteriaceae

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

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