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Sigma-Aldrich

Eosin Y

75% (HPLC)

Synonym(s):

2′,4′,5′,7′-Tetrabromofluorescein, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, Eosin yellowish, Solvent red 43

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About This Item

Empirical Formula (Hill Notation):
C20H8Br4O5
CAS Number:
15086-94-9
Molecular Weight:
647.89
Colour Index Number:
45380:2
EC Number:
239-138-3
MDL number:
MFCD00036189
UNSPSC Code:
12171500
PubChem Substance ID:
24853789
NACRES:
NA.47

Quality Level

100

Assay

75% (HPLC)

form

powder

color

orange to very dark orange

solubility

spirit: soluble

λmax

524 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C24OC(=O)c5ccccc45)c1Br

InChI

1S/C20H8Br4O5/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20/h1-6,25-26H

InChI key

DBZJJPROPLPMSN-UHFFFAOYSA-N

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General description

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

Application

Eosin Y has been used as a reagent in the preparation of Nigrosin-Eosin (NE) stain and is used in hematoxylin and eosin (H&E) staining technique to visualize alginate.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Gregorios J B
Histophatologic Techniques (2006)
Electrophoretic detection of alginate by Hematoxylin and Eosin fluorescence: Implications in cell encapsulation/tissue engineering
Verma Y K
Reactive functional Polymers, 102, 130-136 (2016)
Concordance among pathologists in the second Cardiac Allograft Rejection Gene Expression Observational Study (CARGO II).
Crespo-Leiro MG, Zuckermann A, Bara C, et al.
Transplantation, 94(11), 1172-1177 (2012)
Morphometric dimensions of the spermatozoa in Thai native boar depend onthe ejaculates
Nasinprom N
Animal Reproduction, 540-543 (2015)
John D. Bancroft
Theory and Practice of Histological Techniques (2008)
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Protocols

Determination of Silver in Water and Wastewater: Photometric Determination Using Phenanthroline Method and Eosin Y

The article describes the sample preparation and photometric determination of silver concentration in water samples using Spectroquant® photometers and test kits.

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