222550
Nile Blue chloride
Dye content 85 %
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About This Item
Empirical Formula (Hill Notation):
C20H20ClN3O
CAS Number:
Molecular Weight:
353.85
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
Colour Index Number:
51180
MDL number:
Beilstein/REAXYS Number:
3923952
InChI
1S/C20H19N3O.ClH/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;/h5-12,21H,3-4H2,1-2H3;1H
SMILES string
[Cl-].CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34
InChI key
SHXOKQKTZJXHHR-UHFFFAOYSA-N
form
powder
composition
Dye content, 85%
solubility
water: 1 mg/mL, opaque, blue to very deep blue
λmax
638 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
Related Categories
Application
Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies.
Biochem/physiol Actions
Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9.4) to purple-red (11.0). Nile blue chloride has been used to detect DNA and lipids. In addition, it also helps to identify bacterial growth. Nile blue chloride also facilitates diagnosis of disease linked with amyloid accumulation.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Effect of ?-casein on surface activity of Quillaja bark saponin at fluid/fluid interfaces
Wojciechowski K
Food Hydrocolloids, 34, 208-216 (2014)
R. W. Sabnis
Handbook of Acid-Base Indicators (2007)
Jason D Slinker et al.
Nature chemistry, 3(3), 228-233 (2011-02-22)
Molecular wires show promise in nanoscale electronics, but the synthesis of uniform, long conductive molecules is a significant challenge. Deoxyribonucleic acid (DNA) of precise length, by contrast, is synthesized easily, but its conductivity over the distances required for nanoscale devices
S S Mortazavi et al.
Biological trace element research, 144(1-3), 1430-1436 (2011-07-07)
A simple and sensitive kinetic-spectrophotometric method is developed for the determination of trace amounts of iodine in blood serum samples based on its catalytic effect on the oxidation of Nile Blue A by potassium bromate in sulfuric acid medium and
M Oshiki et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 64(3), 747-753 (2011-11-22)
The present study was conducted (1) to develop a rapid quantification method of polyhydroxyalkanoates (PHA) concentration in activated sludge by Nile blue A staining and fluorescence measurement and (2) to perform on-line monitoring of PHA concentrations in activated sludge. Activated
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