Skip to Content
Merck
CN
All Photos(3)

Documents

216313

Sigma-Aldrich

Methylene Violet (Bernthsen)

certified by the Biological Stain Commission, Dye content ≥65 %

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

Empirical Formula (Hill Notation):
C14H12N2OS
CAS Number:
2516-05-4
Molecular Weight:
256.32
Colour Index Number:
52041
EC Number:
219-733-4
MDL number:
MFCD00005064
UNSPSC Code:
12171500
PubChem Substance ID:
24852949
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

100

form

powder

composition

Dye content, ≥65%

mp

216 °C (dec.) (lit.)

solubility

soluble, clear

λmax

580 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CN(C)c1ccc2N=C3C=CC(=O)C=C3Sc2c1

InChI

1S/C14H12N2OS/c1-16(2)9-3-5-11-13(7-9)18-14-8-10(17)4-6-12(14)15-11/h3-8H,1-2H3

InChI key

ALJHHTHBYJROOG-UHFFFAOYSA-N

Related Categories

Suitability

Certified for use as a constituent of MacNeal′s tetrachrome stain for blood.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

H Morrison et al.
Photochemistry and photobiology, 66(2), 245-252 (1997-08-01)
The interaction of methylene violet (MV) and 4-bromomethylene violet (BrMV) with calf thymus and supercoiled phi X174 phage RF I DNA is reported. Measurements employing UV-visible absorption spectroscopy and equilibrium dialysis give evidence for the formation of complexes by each
A Skripchenko et al.
Photochemistry and photobiology, 65(3), 451-455 (1997-03-01)
Previous studies with methylene blue (MB) in red cell suspensions have demonstrated that extracellular, but not intracellular, virus can be readily photoinactivated. To test if the resistance of intracellular virus to inactivation is related to the permanent positive charge of
C S Sastry et al.
The Analyst, 114(4), 513-515 (1989-04-01)
A fairly sensitive spectrophotometric method for the determination of ibuprofen, ketoprofen, piroxicam, diclofenac sodium, mefenamic acid or enfenamic acid in bulk samples and pharmaceutical preparations is described, based on the formation of a chloroform-soluble, coloured ion-association complex between the drug
Jürgen Burhenne et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 863(2), 273-282 (2008-02-09)
Selective and sensitive methods for the determination of the cationic dye and anti-malarial methylene blue in human liquid whole blood, dried whole blood (paper spot), and plasma depending on protein precipitation and cation exchange chromatography coupled to electrospray ionisation (ESI)
Kevin E Washburn et al.
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 68(2), 105-111 (2004-06-11)
The objectives of this study were to determine the optimal concentration of phenothiazine dye required to inactivate bovine viral diarrhea virus (BVDV) in goat colostrum following 60 min of illumination and determine if immunoglobulin concentration is affected by this technique.
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service