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21022

Supelco

Cesium trifluoroacetate

BioUltra, for molecular biology, ≥99.0% (NT)

Synonym(s):

CsTFA, Trifluoroacetic acid cesium salt

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About This Item

Linear Formula:
CF3COOCs
CAS Number:
21907-50-6
Molecular Weight:
245.92
Beilstein:
4161420
MDL number:
MFCD00051304
UNSPSC Code:
12000000

grade

for molecular biology

product line

BioUltra

Assay

≥99.0% (NT)

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

pH

9.0-10.5 (25 °C, 2 M in H2O)

mp

114-116 °C (lit.)

solubility

H2O: 2 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤500 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤10 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

2 M in H2O

UV absorption

λ: 260 nm Amax: 0.09
λ: 280 nm Amax: 0.04

SMILES string

[Cs+].[O-]C(=O)C(F)(F)F

InChI

1S/C2HF3O2.Cs/c3-2(4,5)1(6)7;/h(H,6,7);/q;+1/p-1

InChI key

RJYSYRSELCQCSO-UHFFFAOYSA-M

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Other Notes

Medium in density gradient centrifugation for the separation of DNA, RNA and protein, and for plasmid isolation; Isolation of mRNA prior to the construction of full-length cDNA libraries

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K Andersson et al.
Plasmid, 13(1), 78-80 (1985-01-01)
Plasmids extracted from bacterial cells by alkaline extraction can easily be isolated from linear DNA by isopycnic centrifugation in CsTFA. This is a fast and simple method which circumvents the use of the intercalating dye, ethidium bromide, and consequently the
D S Zarlenga et al.
Analytical biochemistry, 162(2), 569-574 (1987-05-01)
A rapid method for simultaneously banding preparative amounts of RNA and DNA from Trichinella spiralis muscle larvae by isopycnic centrifugation in cesium trifluoroacetate (CsTFA) is described. Larvae were homogenized in guanidinium isothiocyanate and the DNA, RNA, glycogen, and denatured protein
P E Mirkes
Analytical biochemistry, 148(2), 376-383 (1985-08-01)
A procedure for the simultaneous banding of cellular DNA, RNA, and protein by centrifugation in cesium trifluoroacetate (CsTFA) gradients is described. Starting with homogenates of Day 11 rat embryos, this procedure was used to separate total DNA, RNA, and protein.
High-efficiency cloning of full-length cDNA; construction and screening of cDNA expression libraries for mammalian cells.
H Okayama et al.
Methods in enzymology, 154, 3-28 (1987-01-01)

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