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Key Documents

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198161

Sigma-Aldrich

Toluidine Blue O

certified by the BSC

Synonym(s):

Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride

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100 μL
CN¥5,454.72

CN¥5,454.72

Estimated to ship onMay 19, 2025Details

A recombinant, preservative-free antibody is available for your target. Try ZRB1151
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100 μL
CN¥5,454.72

About This Item

Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
92-31-9
Molecular Weight:
305.83
Colour Index Number:
52040
EC Number:
202-146-2
MDL number:
MFCD00011934
UNSPSC Code:
12171500
PubChem Substance ID:
24851865
NACRES:
NA.47

CN¥5,454.72

Estimated to ship onMay 19, 2025Details

A recombinant, preservative-free antibody is available for your target. Try ZRB1151
Request a Bulk Order

Agency

certified by the BSC

Quality Level

200

form

powder

composition

Dye content, 80%

color

dark green

λmax

626 nm

ε (extinction coefficient)

≥13000 at 241-247 nm in H2O
≥28000 at 287-293 nm in H2O
≥40000 at 625-635 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C

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Show Differences

1 of 4

This Item
179191589572C100307
环己烷 anhydrous, 99.5%

227048

环己烷

环己烷 ACS reagent, ≥99%

179191

环己烷

环己烷 99.5%, anhydrous

589572

环己烷

环己烷 Laboratory Reagent, ≥99.8%

C100307

环己烷

assay

99.5%

assay

≥99%

assay

99.5%

assay

≥99.8%

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

grade

anhydrous

grade

ACS reagent

grade

anhydrous

grade

Laboratory Reagent

form

liquid

form

liquid

form

-

form

liquid

autoignition temp.

500 °F

autoignition temp.

500 °F

autoignition temp.

500 °F

autoignition temp.

500 °F

impurities

<0.001% water, <0.005% water (100 mL pkg)

impurities

H2SO4, passes test (darkened), ≤0.02% water

impurities

-

impurities

-

General description

A metachromatic dye suitable for a wide variety of histological staining procedures.

Application

Toluidine Blue O along with light-emitting diode has been used as a photosensitizer to control in vitro biofilms and to kill gram-negative bacteria.[1][2]

Biochem/physiol Actions

Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls.[3] TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite.[4] TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries.[5] This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin.[6]

Suitability

Certified for use as a metachromatic stain for mast cells and cartilage using the Churukian-Schenk method and the method of Nocht on paraffin sections.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SHBQ4530
    SHBR8115
    SHBP9405
    SHBQ3835
    SHBP2785

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    The Effect of Variable Energy Input from a Novel Light Source on the Photoactivated Bactericidal Action of Toluidine Blue O on Streptococcus mutans
    Williams J A
    Caries Research, 37, 190?193-190?193 (2003)
    Anaīs Noblanc et al.
    Antioxidants (Basel, Switzerland), 9(2) (2020-02-15)
    There is growing evidence that the quality of spermatozoa decreases with age and that children of older fathers have a higher incidence of birth defects and genetic mutations. The free radical theory of aging proposes that changes with aging are
    Polychromatic staining of plant cell walls by toluidine blue O
    Brien T P O
    Protoplasma, 59(2), 368- 373 (1964)
    The adsorption kinetics and removal of cationic dye, Toluidine Blue O, from aqueous solution with Turkish zeolite
    Alpat S K
    Journal of Hazardous Materials, 151(1), 213-220 (2008)
    Factors influencing the susceptibility of Gram-negative bacteria to toluidine blue O-mediated lethal photosensitization
    komerik N
    Journal of Applied Microbiology, 92(4), 618?623-618?623 (2002)
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