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Ponceau 4R

analytical standard

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Synonym(s):
New Coccine, Acid Red 18, Ponceau 4 R
Empirical Formula (Hill Notation):
C20H11N2Na3O10S3
CAS Number:
2611-82-7
Molecular Weight:
604.47
Colour Index Number:
16255
Beilstein:
4122340
EC Number:
220-036-2
MDL number:
MFCD00004084
UNSPSC Code:
12164500
PubChem Substance ID:
329751631
E Number:
E124
NACRES:
NA.24

grade

analytical standard

Quality Level

100

Assay

≥98.0% (HPLC)

shelf life

limited shelf life, expiry date on the label

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

application(s)

cleaning products
cosmetics
food and beverages
personal care

format

neat

SMILES string

[Na+].[Na+].[Na+].Oc1ccc2cc(cc(c2c1\N=N\c3ccc(c4ccccc34)S([O-])(=O)=O)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C20H14N2O10S3.3Na/c23-16-7-5-11-9-12(33(24,25)26)10-18(35(30,31)32)19(11)20(16)22-21-15-6-8-17(34(27,28)29)14-4-2-1-3-13(14)15;;;/h1-10,23H,(H,24,25,26)(H,27,28,29)(H,30,31,32);;;/q;3*+1/p-3/b22-21+;;;

InChI key

SWGJCIMEBVHMTA-ZRUFZDNISA-K

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General description

Ponceau 4R is a sulfonated azo dye, used as a food colorant to give red color to different food products, such as alcoholic beverages, soft drinks, bakery products, candies, syrups, preserved fruits, etc. An acceptable daily intake (ADI) of 0.4 to 0.7 mg (kg body weight)-1 was set for Ponceau 4R by the European Food Safety Authority (EFSA) in 2009.

Application

The analytical standards can also be used as follows:
  • Spectrophotometric quantitative estimation of Brilliant Blue, Sunset Yellow, Ponceau 4R, and Tartrazin from juice samples, using a transparent polymer matrix for extraction
  • Liquid-liquid microextraction of five synthetic food dyes from different food samples for their determination by high-performance liquid chromatography with a variable wavelength ultraviolet detector (HPLC-VWUVD)
  • Simultaneous analysis of five water-soluble synthetic colorants in different food samples by on-plate solid-phase extraction and thin-layer chromatography (TLC) with backlight-assisted detection
  • Multi-residue determination of 11 synthetic color food additives in high protein and fat content food products by HPLC combined with a diode array detector (DAD) or a tandem mass spectrometer (MS/MS)
  • Extraction of eight synthetic food dyes from 30 food samples of six types using an NH2-aminopropyl-modified silica SPE cartridge for their quantification by RP-HPLC coupled with UV detector

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Genotoxicity of Synthetic Food Colorants
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Analysis of methanol-ethanol mixtures from falsified beverages using a dual biosensors amperometric system based on alcohol dehydrogenase and alcohol oxidase.
Bucur, Bogdan, Gabriel Lucian Radu, and Constantin Nicolae Toader
European Food Research and Technology, 226, 1335-1342 (2008)
Encyclopedia of Food Safety, 640-640 (2013)
Priyanka Bolel et al.
Journal of agricultural and food chemistry, 60(14), 3727-3734 (2012-03-09)
Cochineal Red A is a negatively charged synthetic azo food colorant and a potential carcinogen. We present here the study of binding of Cochineal Red A with two homologous serum albumins, human (HSA) and bovine (BSA), in aqueous pH 7.4
Chih-Huang Weng et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 62(4), 844-851 (2010-08-24)
The ability of magnetic Fe₃O₄ nanoparticles (MFN) to remove new coccine (NC), an acidic dye, from aqueous solutions was studied. Parameters including ionic strength, pH, and temperature were evaluated. MFN, prepared by precipitation method, exhibits an average particle size of
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