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125660

Sigma-Aldrich

Leucomalachite Green

powder

Synonym(s):

4,4′-Benzylidenebis(N,N-dimethylaniline)

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About This Item

Linear Formula:
C6H5CH[C6H4N(CH3)2]2
CAS Number:
129-73-7
Molecular Weight:
330.47
Beilstein:
2140506
EC Number:
204-961-9
MDL number:
MFCD00008315
UNSPSC Code:
12171500
PubChem Substance ID:
24847677
NACRES:
NA.47

form

powder

Quality Level

100

mp

100-102 °C (lit.)

λmax

623 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CN(C)c1ccc(cc1)C(c2ccccc2)c3ccc(cc3)N(C)C

InChI

1S/C23H26N2/c1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4/h5-17,23H,1-4H3

InChI key

WZKXBGJNNCGHIC-UHFFFAOYSA-N

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Application

Leucomalachite green has been used as a reagent in presumptive tests which are used to detect invisible blood stains.

Biochem/physiol Actions

Leucomalachite green (LMG) is a chief metabolite of malachite green, a triphenylmethane dye that has been used generally as an antifungal drug in the fish industry. DNA adducts formed in livers of rats fed with leucomalachite green have been observed with little mutagenic or carcinogenic effect. LMG is one of the most commonly used reagents for presumptive blood test. It gives a characteristic green color in the presence of blood. LMG destroys DNA in the blood, therefore, the chances of DNA recovery and amplification is reduced or eliminated.

Signal Word

Warning

Hazard Statements

H302,H341,H351,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Muta. 2

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Mutagenicity and carcinogenicity in relation to DNA adduct formation in rats fed leucomalachite green.
Culp SJ
Mutation Research, 506-507, 55-63 (2002)
Evidence of aerosolised floating blood mist during oral surgery.
Ishihama K
Journal of Hospital Infection, 71(4), 359-364 (2009)
Kevin J Farrugia et al.
Science & justice : journal of the Forensic Science Society, 51(3), 110-121 (2011-09-06)
This study investigates the optimisation of peroxidase based enhancement techniques for footwear impressions made in blood on various fabric surfaces. Four different haem reagents: leuco crystal violet (LCV), leuco malachite green (LMG), fluorescein and luminol were used to enhance the
Jun Bae Lee et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 27(7), 953-961 (2010-06-15)
This paper presents analysis of malachite green (MG) and crystal violet (CV) residues in processed fish products. Samples were homogenized and extracted with ammonium acetate buffer and acetonitrile. The extracted residues were partitioned into dichloromethane, in situ oxidized to chromic
Sara L Stead et al.
Analytical chemistry, 82(7), 2652-2660 (2010-03-06)
A robust screening assay employing solid phase extraction (SPE) followed by a novel aptamer-based procedure is presented for the rapid detection and semiquantitation of the triphenylmethane dye, Malachite Green (MG) and its primary metabolite Leucomalachite Green (LMG) in fish tissue.
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