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12125

Sigma-Aldrich

Benzidine dihydrochloride

for experimental carcinogenesis, ≥99.0%

Synonym(s):

4,4′-Diaminobiphenyl dihydrochloride

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About This Item

Empirical Formula (Hill Notation):
C12H12N2 · 2HCl
CAS Number:
531-85-1
Molecular Weight:
257.16
Beilstein:
3914846
EC Number:
208-519-6
MDL number:
MFCD00040389
UNSPSC Code:
12352207
PubChem Substance ID:
329749630
NACRES:
NA.25

grade

for experimental carcinogenesis

Quality Level

100

Assay

≥99.0% (AT)
≥99.0%

form

solid

ign. residue

≤0.05%

mp

≥300 °C

SMILES string

Cl[H].Cl[H].Nc1ccc(cc1)-c2ccc(N)cc2

InChI

1S/C12H12N2.2ClH/c13-11-5-1-9(2-6-11)10-3-7-12(14)8-4-10;;/h1-8H,13-14H2;2*1H

InChI key

RUAXWVDEYJEWRY-UHFFFAOYSA-N

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Application

Benzidine dihydrochloride (BDHC) may be used as a chromogenic substrate for the detection of horseradish peroxidase in immunochemical and immunohistochemical applications.

Warning

Cancer suspect agent.

Other Notes

Indicator of carcinogenicity in bioassay studies; For the detection of horseradish peroxidase in histochemical studies
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Signal Word

Danger

Hazard Statements

H302,H350,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1A

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
WE314499
314499/1
WE314499V
WE314499

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C.H. Frith et al.
Toxicology Letters, 4, 507-507 (1979)
S Lakos et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 34(8), 1047-1056 (1986-08-01)
Although very sensitive chromogens have been adapted for localization of horseradish peroxidase in anterograde and retrograde tracing studies, they have not been successfully applied in immunocytochemical studies. This report describes a protocol which uses benzidine dihydrochloride (BDHC) as the chromogen
M M Mesulam et al.
Brain research, 136(3), 393-414 (1977-11-18)
The caudal part of the inferior parietal lobule (area PG) was injected with horseradish peroxidase (HRP) in 6 hemispheres of 5 rhesus monkeys. The retrograde transport of HRP resulted in the labeling of neurons in diverse cortical and subcortical areas.
Ping Lin et al.
Molecular cell, 78(5), 850-861 (2020-04-30)
Cas13 has demonstrated unique and broad utility in RNA editing, nucleic acid detection, and disease diagnosis; however, a constantly active Cas enzyme may induce unwanted effects. Bacteriophage- or prophage-region-encoded anti-CRISPR (acr) gene molecules provide the potential to control targeting specificity
M M Mesulam et al.
Neuroscience letters, 5(1-2), 7-14 (1977-06-01)
Four rhesus monkeys and two rats were injected with horseradish peroxidase (HRP). Adjacent sections were stained with a 3,3'-diaminobenzidine tetrahydrochloride (DAB) and a benzidine dihydrochloride (BDHC) procedure which yields a blue reaction product at sites of HRP activity. The number
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