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114405

Sigma-Aldrich

Bromophenol Blue sodium salt

Dye content 90 %, ACS reagent

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Synonym(s):
3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt
Empirical Formula (Hill Notation):
C19H9Br4NaO5S
CAS Number:
34725-61-6
Molecular Weight:
691.94
Beilstein:
6995701
EC Number:
252-170-2
MDL number:
MFCD00013793
UNSPSC Code:
12171500
PubChem Substance ID:
24847170
NACRES:
NA.47

Agency

suitable for SM 2310
suitable for SM 2320

Quality Level

200

form

powder

composition

Dye content, 90%

protein detection level measuring range

10-80 μg

technique(s)

titration: suitable

color

blue, at pH above 4.6

visual transition interval

3.0-4.6, yellow to blue

λmax

383 nm
589 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

InChI key

TWKDJMRHSLWRFI-UHFFFAOYSA-M

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Application

Bromophenol Blue has been used in the sample buffer prepared for western blotting.
A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Determination of VPS34/PIK3C3 Activity in vitro Utilising 32P-?ATP.
Munson MJ, et al.
Bio-protocol null
Jinjiang Li et al.
Journal of pharmaceutical sciences, 93(3), 697-712 (2004-02-06)
In this study, we have examined the acid-base characteristics of various citrate buffer systems alone and in the presence of the pH indicator dye, bromophenol blue, in aqueous solution, and after lyophilization to produce amorphous material. Fourier transform Raman and
Ronan C O'Malley et al.
Methods in molecular biology (Clifton, N.J.), 1284, 323-342 (2015-03-12)
The T-DNA sequence-indexed mutant collections contain insertional mutants for most Arabidopsis thaliana genes and have played an important role in plant biology research for almost two decades. By providing a large source of mutant alleles for in vivo characterization of

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