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114375

Sigma-Aldrich

Bromocresol Purple

BioReagent, suitable for indicator, Dye content 90 %

Synonym(s):

5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form

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About This Item

Empirical Formula (Hill Notation):
C21H16Br2O5S
CAS Number:
115-40-2
Molecular Weight:
540.22
Beilstein:
359618
EC Number:
204-087-8
MDL number:
MFCD00011681
UNSPSC Code:
12171500
PubChem Substance ID:
24847168
NACRES:
NA.47

product line

BioReagent

Quality Level

200

Assay

≥89.0% (HPLC)

form

powder

composition

Dye content, 90%

technique(s)

titration: suitable

pH

5.2-6.8, yellow to purple

visual transition interval

5.2-6.8, yellow to blue

mp

240 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL

λmax

419 nm

ε (extinction coefficient)

≥22000 at 418-424 nm in methanol

suitability

suitable for indicator

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4

InChI

1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3

InChI key

ABIUHPWEYMSGSR-UHFFFAOYSA-N

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Application

Bromocresol Purple has been used for staining of Saccharomyces cerevisiae.
Spectrophotometric determination of serum-albumin

Biochem/physiol Actions

Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity.
Kurzweilova H and Sigler K
Yeast, 9, 1207-1211 (1993)
ATP leakage from yeast cells treated by extracellular glycolipids of Pseudozyma fusiformata.
Kulakovskaya TV, et al.
FEMS Yeast Research, 3, 401-404 (2003)
Yeast colonies synchronise their growth and development.
Palkova Z and Forstova J
Journal of Cell Science, 113, 1923-1928 (2000)
André Klauss et al.
Photosynthesis research, 102(2-3), 499-509 (2009-03-31)
Determination of thermodynamic parameters of water oxidation at the photosystem II (PSII) manganese complex is a major challenge. Photothermal beam deflection (PBD) spectroscopy determines enthalpy changes (ΔH) and apparent volume changes which are coupled with electron transfer in the S-state
C H White et al.
Journal of dairy science, 89(7), 2459-2464 (2006-06-15)
The MicroFoss method was evaluated for its effectiveness as an indicator of fluid milk shelf life. Half-gallon, 2% fat fluid milk samples (n = 90) were obtained from a milk processing plant on 3 occasions postpasteurization and evaluated for shelf
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