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Merck
CN

114375

Bromocresol Purple

BioReagent, suitable for indicator, Dye content 90 %

Synonym(s):

5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form

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About This Item

Empirical Formula (Hill Notation):
C21H16Br2O5S
CAS Number:
115-40-2
Molecular Weight:
540.22
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24847168
EC Number:
204-087-8
Beilstein/REAXYS Number:
359618
MDL number:
MFCD00011681

Key Documents

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Product Name

Bromocresol Purple, BioReagent, suitable for indicator, Dye content 90 %

InChI key

ABIUHPWEYMSGSR-UHFFFAOYSA-N

InChI

1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3

SMILES string

Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4

product line

BioReagent

assay

≥89.0% (HPLC)

form

powder

composition

Dye content, 90%

technique(s)

titration: suitable

pH

5.2-6.8, yellow to purple

visual transition interval

5.2-6.8, yellow to blue

mp

240 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL

λmax

419 nm

ε (extinction coefficient)

≥22000 at 418-424 nm in methanol

suitability

suitable for indicator

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Bromocresol Purple has been used for staining of Saccharomyces cerevisiae.
Spectrophotometric determination of serum-albumin

Biochem/physiol Actions

Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000504927
0000504927
SHBT0708
0000485392
0000485392

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Yeast colonies synchronise their growth and development.
Palkova Z and Forstova J
Journal of Cell Science, 113, 1923-1928 (2000)
ATP leakage from yeast cells treated by extracellular glycolipids of Pseudozyma fusiformata.
Kulakovskaya TV, et al.
FEMS Yeast Research, 3, 401-404 (2003)
Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity.
Kurzweilova H and Sigler K
Yeast, 9, 1207-1211 (1993)
Faizah Mohd Faizul et al.
Journal of photochemistry and photobiology. B, Biology, 90(1), 1-7 (2007-11-21)
The interaction between bromocresol purple (BCP) and bovine serum albumin (BSA)/porcine serum albumin (PSA) was investigated both in the absence and presence of bilirubin (BR) using absorption/absorption difference spectroscopy. A significant red shift in the absorption maxima of BCP accompanied
Munzer Kattan et al.
Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine, 69(2), 377-380 (2010-11-27)
Polyvinyl chloride (PVC) dyed with bromocresol purple was investigated as a high-dose radiation dosimeter. The absorbance at 417 nm depends linearly on the dose below 50 kGy. The response depends neither on dose rate nor on the irradiation temperature. The
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