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Sigma-Aldrich

Bromocresol Green sodium salt

90% (HPLC), ACS reagent, Dye content 90 %

Synonym(s):

3′,3″,5′,5″-Tetrabromo-m-cresolsulfonphthalein sodium salt

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About This Item

Empirical Formula (Hill Notation):
C21H13Br4NaO5S
CAS Number:
Molecular Weight:
720.00
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

grade

ACS reagent

Quality Level

Agency

suitable for SM 2310
suitable for SM 2320

Assay

90% (HPLC)

form

powder or crystals

composition

Dye content, 90%

visual transition interval

3.8, yellow(Visual Interval Pass)
3.8-5.2, yellow to blue
4.5, green
5.4, blue(Visual Interval Pass)

mp

230 °C (dec.) (lit.)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Cc1c(Br)c(O)c(Br)cc1\C(=C2\C=C(Br)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O

InChI

1S/C21H14Br4O5S.Na/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21;/h3-8,26-27H,1-2H3;/q;+1/p-1

InChI key

HEFSGAHJDGZCHA-UHFFFAOYSA-M

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General description

Bromocresol Green (BCG), also known as bromcresol green, is a synthetic, anionic, triphenylmethane dye belonging to the phthalein and sulphonphthalein dyes group. It is commonly used as a pH indicator as it changes color from yellow to blue-green at pH 3.8-5.4. The monosodium salt of bromocresol green is highly ionizable in water. The dye at a higher pH level produces a large and weakly lipophilic dianion.

Application

Bromocresol green serves as a pH indicator in biology and medicinal experiments.
  • It is used widely as a vital stain to study blood-brain permeability.
  • It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and in charge-transfer complexation processes.
  • It is used in TLC for the visualization of compounds with functional groups whose pKa is below 5.0.
  • It has been used in the pH-sensitive colorimetric determination of glutamate decarboxylase activity.
  • It has also been employed in the evaluation of paper-analytical devices for colorimetric analysis of serum samples.
  • It is suitable as a selective inhibitor in kidney physiology studies.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Conn?s Biological Stains: A Handbook of Dyes, Stains and Fluorochromes for Use in Biology and Medicine (10th ed.)
RW Horobin, JA Kiernan (Eds.)
Conn?s Biological Stains (2002)
A high-throughput colorimetric assay to measure the activity of glutamate decarboxylase
Yu, et al.
Enzyme and Microbial Technology, 49(3) (2011)
Azo-functionalized superparamagnetic Fe3O4 nanoparticles: an efficient adsorbent for the removal of bromocresol green from contaminated water
Saad, et al.
Royal Society of Chemistry Advances, 12 (2022)
Preparation of Chitosan Poly(methacrylate) Composites for Adsorption of Bromocresol Green
Liu, et al.
ACS Omega, 4(7) (2019)
Martina O' Toole et al.
Talanta, 66(5), 1340-1344 (2008-10-31)
A novel inexpensive optical-sensing technique has been developed for colorimetric flow analysis. This sensing system employs two LEDs whereby one is used as the light source and the other as a light detector. The LED used as light detector is

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