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Merck
CN

114359

Bromocresol Green

ACS reagent, Dye content 95 %

Synonym(s):

BCG; 2,6-dibromo-4-[3-(3,5-dibromo-4-hydroxy-2-methylphenyl)-1,1-dioxo-2,1λ6-benzoxathiol-3-yl]-3-methylphenol

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About This Item

Empirical Formula (Hill Notation):
C21H14Br4O5S
CAS Number:
76-60-8
Molecular Weight:
698.01
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24847166
EC Number:
200-972-8
Beilstein/REAXYS Number:
372527
MDL number:
MFCD00005874

Key Documents

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Product Name

Bromocresol Green, ACS reagent, Dye content 95 %

Quality Level

200

storage temp.

room temp

InChI key

FRPHFZCDPYBUAU-UHFFFAOYSA-N

InChI

1S/C21H14Br4O5S/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21/h3-8,26-27H,1-2H3

SMILES string

Cc1c(Br)c(O)c(Br)cc1C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4C

grade

ACS reagent

assay

≥95% (After Oxygen Combustion, silver nitrate titration)

form

powder

composition

Dye content, 95%

visual transition interval

3.8, yellow(passes test)
3.8-5.4, yellow to blue(passes test)
4.5, green
5.4, blue

mp

225 °C (dec.) (lit.)

λmax

423 nm

application(s)

diagnostic assay manufacturing
hematology
histology

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Application

Bromocresol green serves as a pH indicator in biology and medicinal experiments.
  • It is used widely as a vital stain to study blood-brain permeability.
  • It has been used in the pH-sensitive colorimetric determination of glutamate decarboxylase activity.
  • It has also been employed in the evaluation of paper-analytical devices for colorimetric analysis of serum samples.
  • It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and in charge-transfer complexation processes.
  • It is used in TLC for the visualization of compounds with functional groups whose pKa is below 5.0.

General description

Bromocresol Green is a synthetic triphenylmethane dye that is anionic in nature. At pH 3.8-5.4, it changes color from yellow to blue-green, and is therefore, commonly used as a pH indicator.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000520877
0000520877
SHBT1324
0000438815
0000438815

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Selection of appropriate protein assay method for a paper microfluidics platform
Pokhrel, et al.
practical laboratory medicine, 21 (2020)
A high-throughput colorimetric assay to measure the activity of glutamate decarboxylase
Yu, et al.
Enzyme and Microbial Technology, 49 (2011)
Conn?s Biological Stains: A Handbook of Dyes, Stains and Fluorochromes for Use in Biology and Medicine (10th ed.)
Horobin, R. W., & Kiernan, J. A. (Eds.)
Conn?s Biological Stains (2002)
Preparation of Chitosan Poly(methacrylate) Composites for Adsorption of Bromocresol Green
Liu, et al.
ACS Omega, 4 (2019)
Fanya Ismail et al.
The Analyst, 136(4), 807-815 (2010-12-02)
In this paper we present a new theory to re-examine the immobilization technique of dye doped sol-gel films, define the strength and types of possible bonds between the immobilized molecule and sol-gel glass, and show that the immobilized molecule is
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