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Sigma-Aldrich

Bromocresol Green

ACS reagent, Dye content 95 %

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Synonym(s):
BCG; 2,6-dibromo-4-[3-(3,5-dibromo-4-hydroxy-2-methylphenyl)-1,1-dioxo-2,1λ6-benzoxathiol-3-yl]-3-methylphenol
Empirical Formula (Hill Notation):
C21H14Br4O5S
CAS Number:
76-60-8
Molecular Weight:
698.01
Beilstein:
372527
EC Number:
200-972-8
MDL number:
MFCD00005874
UNSPSC Code:
12171500
PubChem Substance ID:
24847166
NACRES:
NA.47

grade

ACS reagent

Quality Level

200

Assay

≥95% (After Oxygen Combustion, silver nitrate titration)

form

powder

composition

Dye content, 95%

visual transition interval

3.8, yellow(passes test)
3.8-5.4, yellow to blue(passes test)
4.5, green
5.4, blue

mp

225 °C (dec.) (lit.)

λmax

423 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cc1c(Br)c(O)c(Br)cc1C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4C

InChI

1S/C21H14Br4O5S/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21/h3-8,26-27H,1-2H3

InChI key

FRPHFZCDPYBUAU-UHFFFAOYSA-N

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General description

Bromocresol Green is a synthetic triphenylmethane dye that is anionic in nature. At pH 3.8-5.4, it changes color from yellow to blue-green, and is therefore, commonly used as a pH indicator.

Application

Bromocresol green serves as a pH indicator in biology and medicinal experiments.
  • It is used widely as a vital stain to study blood-brain permeability.
  • It has been used in the pH-sensitive colorimetric determination of glutamate decarboxylase activity.
  • It has also been employed in the evaluation of paper-analytical devices for colorimetric analysis of serum samples.
  • It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and in charge-transfer complexation processes.
  • It is used in TLC for the visualization of compounds with functional groups whose pKa is below 5.0.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Preparation of Chitosan Poly(methacrylate) Composites for Adsorption of Bromocresol Green
Liu, et al.
ACS Omega, 4 (2019)
Selection of appropriate protein assay method for a paper microfluidics platform
Pokhrel, et al.
practical laboratory medicine, 21 (2020)
Conn?s Biological Stains: A Handbook of Dyes, Stains and Fluorochromes for Use in Biology and Medicine (10th ed.)
Horobin, R. W., & Kiernan, J. A. (Eds.)
Conn?s Biological Stains (2002)
Martina O' Toole et al.
Talanta, 66(5), 1340-1344 (2008-10-31)
A novel inexpensive optical-sensing technique has been developed for colorimetric flow analysis. This sensing system employs two LEDs whereby one is used as the light source and the other as a light detector. The LED used as light detector is
Fanya Ismail et al.
The Analyst, 136(4), 807-815 (2010-12-02)
In this paper we present a new theory to re-examine the immobilization technique of dye doped sol-gel films, define the strength and types of possible bonds between the immobilized molecule and sol-gel glass, and show that the immobilized molecule is
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