10831
D-Amino acid Oxidase, immobilized from Trigonopsis variabilis
powder (moist), orange-brown, ~20 U/g
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Certificates of Analysis (COA)
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Are there limitations when attempting to use Product 10831, Histopaque®-1083 with the various Accuspin products?
Yes. There are some limitations. Small amounts of blood cannot be processed in the Accuspin tubes. At least 3 mls of whole peripheral blood is expected. Without sufficient red cell numbers, the mononuclear band fails to adequately clear the barrier or frit found within the Accuspin tubes.
I find mononuclear cells separate well from human blood over Product 10831, Histopaque®-1083, but the cell recovery from rats and mice is lower. Why?
The most common cause for separation problems with Histopaque® 1083 is the blood source. Blood from rats and mice is harder to obtain than blood from a human. Most users experiencing problems are collecting blood with a syringe. It is relatively uncommon for users to call in about poor separation when using vacuum tubes. Humanely sacrifice the animal if possible and immediately collect the blood from the aorta or vena cava. Collecting blood from a severed head is never acceptable. Blood obtained from bleeding the tail is also generally unacceptable. Tissue fluids and other extraneous debris will often result in an activation of the coagulation cascase. Blood drawn into a vacuum tube is the best source of blood. See the product insert for Histopaque® 1083 for the amount of anticoagulant to add to blood collected with a syringe.
Can Product 10831, Histopaque®-1083, be used with human blood?
Cells can be recovered from human blood using Histopaque® 1083. However Sigma sells Histopaque® 1077 specifically for use with human blood. Histopaque® 1077 is intended for In Vitro Diagnostic Use. Histopaque® 1083 is intended for research use only with animal blood.
Is there any data for expected percent recovery with rat or mouse blood when using Product 10831, Histopaque®-1083?
No. Histopaque® 1083 is a research use product and no studies have been performed to determine percent recovery.
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