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09709

Sigma-Aldrich

Ammonium phosphate monobasic

BioUltra, ≥99.5% (T)

Synonym(s):

mono-Ammonium phosphate, prim-Ammonium phosphate, Ammonium dihydrogenphosphate

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About This Item

Linear Formula:
NH4H2PO4
CAS Number:
Molecular Weight:
115.03
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

product line

BioUltra

Quality Level

Assay

≥99.5% (T)

impurities

insoluble matter, passes filter test

pH

4.0-5.0 (25 °C, 0.1 M in H2O)

mp

190 °C (dec.) (lit.)

solubility

H2O: 0.1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.5 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤50 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤20 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.1 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.059
λ: 280 nm Amax: ≤0.050

SMILES string

N.OP(O)(O)=O

InChI

1S/H3N.H3O4P/c;1-5(2,3)4/h1H3;(H3,1,2,3,4)

InChI key

LFVGISIMTYGQHF-UHFFFAOYSA-N

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Application

Ammonium phosphate monobasic can be used to study optimization reagents, protein structural analysis, proteomics, x-ray crystallography and biological buffers. Ammonium phosphate monobasic has been used to develop a simple high-performance liquid chromatographic method for determining N1-alkylnicotinamides, including C1C5 alkyl derivatives, in urine. Ammonium phosphate monobasic has also been used to develop a high-performance liquid chromatographic assay coupled with UV detection (254 nm) for the determination of midazolam and two of its hydroxylated metabolites in human plasma.

Other Notes

Component of culture media e.g. DEV SIMMONS Citrate agar. Acetate agar acc. to Trabulsi and Ewing

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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L.R. Trabulsi et al.
Publ. Hlth. Lab., 20, 137-137 (1962)
T Hirayama et al.
Analytical biochemistry, 147(1), 108-113 (1985-05-15)
A simple high-performance liquid chromatographic method has been developed for determining N1-alkylnicotinamides, including C1-C5 alkyl derivatives, in urine. N1-Alkylnicotinamides were reacted with acetophenone in strong alkali medium at 0 degrees C and then formic acid was added. The reaction mixture
María J Nozal et al.
Journal of pharmaceutical and biomedical analysis, 30(2), 285-291 (2002-08-23)
A high performance liquid chromatographic (HPLC) method for the assay of sumatriptan succinate residues in swabs collected from manufacturing equipment surfaces was developed and validated in order to control a cleaning procedure. The swabbing procedure using two cotton swabs moistened
Xiaopeng Gao et al.
Journal of the science of food and agriculture, 90(5), 750-758 (2010-04-01)
A 3-year field trial was conducted to investigate the effect of agricultural management practices including tillage, preceding crop and phosphate fertilization on root colonization by arbuscular mycorrhizal (AM) fungi and grain cadmium (Cd) concentration of durum wheat (Triticum turgidum L.).
Everest Akaraonye et al.
Biotechnology journal, 7(2), 293-303 (2011-12-08)
The main hindrance in the use of polyhydroxyalkanoates (PHAs) as a replacement for existing petroleum-based plastics is their high production cost. The carbon source accounts for 50% of the cost for PHA production. Thus, increasing the yield and productivity of

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