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03334

Supelco

Dansylhydrazine

for LC-MS derivatization, LiChropur™, ≥95% (HPLC)

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Synonym(s):
5-(Dimethylamino)-1-naphthalenesulfonic hydrazide, 5-(Dimethylamino)naphthalene-1-sulfono­hydra­zide, Dns-Hz
Linear Formula:
(CH3)2NC10H6SO2NHNH2
CAS Number:
33008-06-9
Molecular Weight:
265.33
Beilstein:
2868662
EC Number:
251-337-7
MDL number:
MFCD00003986
UNSPSC Code:
41116105
PubChem Substance ID:
329747590
NACRES:
NA.22

Quality Level

100

Assay

≥95% (HPLC)

quality

LiChropur™

technique(s)

LC/MS: suitable

mp

126-130 °C (lit.)

suitability

conforms to structure for Infrared spectrum

SMILES string

CN(C)c1cccc2c(cccc12)S(=O)(=O)NN

InChI

1S/C12H15N3O2S/c1-15(2)11-7-3-6-10-9(11)5-4-8-12(10)18(16,17)14-13/h3-8,14H,13H2,1-2H3

InChI key

KPQYDVAFRDWIBW-UHFFFAOYSA-N

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General description

Dansylhydrazine (DNSH) is a fluorescent reagent which is commonly utilized for derivatization of carbonyl compounds.

Application

Suitable for LC-MS derivatization.
DNSH has been used for the analytical derivatization of malondialdehyde prior to its determination in human plasma by liquid chromatography-mass spectrometry (LC-MS).
It may be used as a derivatization agent for thujone prior to its determination in white Italian vermouth samples by reversed phase LC with fluorescence detection combined with tandem MS and as a fluorescent label for the quantification of neuroactive steroids, alphaxalone and pregnanolone in plasma by isocratic reversed-phase high-performance liquid chromatography (RP-HPLC).

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Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Precautionary Statements

P261 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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High-performance liquid chromatography of the neuroactive steroids alphaxalone and pregnanolone in plasma using dansyl hydrazine as fluorescent label: application to a pharmacokinetic-pharmacodynamic study in rats.
Visser, et al.
Journal of Chromatography. B, Biomedical Applications, 745(2), 357-363 (2000)
Determination of malondialdehyde in human plasma by fully automated solid phase analytical derivatization.
Lord, et al.
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 877(13), 1292-1298 (2009)
Determination of thujone by derivatization with dansylhydrazine and liquid chromatography.
Scott, et al.
Journal of Liquid Chromatography and Related Technologies, 27(6), 1071-1081 (2004)
Tomofumi Santa
Biomedical chromatography : BMC, 25(1-2), 1-10 (2010-11-09)
Liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) is one of the most prominent analytical techniques owing to its inherent selectivity and sensitivity. In LC/ESI-MS/MS, chemical derivatization is often used to enhance the detection sensitivity. Derivatization improves the chromatographic separation, and
S A Visser et al.
Journal of chromatography. B, Biomedical sciences and applications, 745(2), 357-363 (2000-10-24)
This report describes a rapid and sensitive analytical method for the quantification of the neuroactive steroids alphaxalone and pregnanolone in rat plasma using derivatization with dansyl hydrazine as fluorescent label. The method involves protein precipitation, alkaline derivatization and extraction of

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