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Merck
CN

03334

Dansylhydrazine

for LC-MS derivatization, LiChropur™, ≥95% (HPLC)

Synonym(s):

5-(Dimethylamino)-1-naphthalenesulfonic hydrazide, 5-(Dimethylamino)naphthalene-1-sulfono­hydra­zide, Dns-Hz

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About This Item

Linear Formula:
(CH3)2NC10H6SO2NHNH2
CAS Number:
33008-06-9
Molecular Weight:
265.33
UNSPSC Code:
41116105
NACRES:
NA.22
PubChem Substance ID:
329747590
EC Number:
251-337-7
Beilstein/REAXYS Number:
2868662
MDL number:
MFCD00003986

Key Documents

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InChI

1S/C12H15N3O2S/c1-15(2)11-7-3-6-10-9(11)5-4-8-12(10)18(16,17)14-13/h3-8,14H,13H2,1-2H3

InChI key

KPQYDVAFRDWIBW-UHFFFAOYSA-N

SMILES string

CN(C)c1cccc2c(cccc12)S(=O)(=O)NN

assay

≥95% (HPLC)

quality

LiChropur™

technique(s)

LC/MS: suitable

mp

126-130 °C (lit.)

suitability

conforms to structure for Infrared spectrum

Quality Level

100

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General description

Dansylhydrazine (DNSH) is a fluorescent reagent which is commonly utilized for derivatization of carbonyl compounds.

Application

Suitable for LC-MS derivatization.
DNSH has been used for the analytical derivatization of malondialdehyde prior to its determination in human plasma by liquid chromatography-mass spectrometry (LC-MS).
It may be used as a derivatization agent for thujone prior to its determination in white Italian vermouth samples by reversed phase LC with fluorescence detection combined with tandem MS and as a fluorescent label for the quantification of neuroactive steroids, alphaxalone and pregnanolone in plasma by isocratic reversed-phase high-performance liquid chromatography (RP-HPLC).

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCB8488
BCBR7159V

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Determination of malondialdehyde in human plasma by fully automated solid phase analytical derivatization.
Lord, et al.
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 877(13), 1292-1298 (2009)
High-performance liquid chromatography of the neuroactive steroids alphaxalone and pregnanolone in plasma using dansyl hydrazine as fluorescent label: application to a pharmacokinetic-pharmacodynamic study in rats.
Visser, et al.
Journal of Chromatography. B, Biomedical Applications, 745(2), 357-363 (2000)
Determination of thujone by derivatization with dansylhydrazine and liquid chromatography.
Scott, et al.
Journal of Liquid Chromatography and Related Technologies, 27(6), 1071-1081 (2004)
Tomofumi Santa
Biomedical chromatography : BMC, 25(1-2), 1-10 (2010-11-09)
Liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) is one of the most prominent analytical techniques owing to its inherent selectivity and sensitivity. In LC/ESI-MS/MS, chemical derivatization is often used to enhance the detection sensitivity. Derivatization improves the chromatographic separation, and
S A Visser et al.
Journal of chromatography. B, Biomedical sciences and applications, 745(2), 357-363 (2000-10-24)
This report describes a rapid and sensitive analytical method for the quantification of the neuroactive steroids alphaxalone and pregnanolone in rat plasma using derivatization with dansyl hydrazine as fluorescent label. The method involves protein precipitation, alkaline derivatization and extraction of

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