Universal ProbeLibrary

Two vials, each containing 125 μL of one probe solution [10 μM solution of a single probe, 5′ labeled with fluorescein (FAM) and 3′ labeled with a dark quencher dye].

Probe chemistry: Universal ProbeLibrary probes use a unique nucleotide chemistry called LNA (Locked Nucleic Acid), which allows very short (8-9 bases) oligonucleotides to be effective hybridization probes in real-time PCR assays. LNA allows the melting temperature of the short probes to be unusually high. Thus, they are fully compatible with commonly used PCR conditions and the hydrolysis-probe detection format. These probes are even sensitive enough to detect single-base mismatches.

Assay design: The free online ProbeFinder software, available at the Universal ProbeLibrary Assay Design Center, designs one or more intron-spanning assays for a target gene, based on specific information (i.e., gene name, accession number, or sequence) that you submit. For each assay it designs, the software will specify a set of specific primers plus a probe from the Universal ProbeLibrary that will give the best results. The combination of primers and probe will provide specific amplification and detection of your target sequence in a standard real-time PCR assay.

Labeling: pre-labeled with a reporter fluorophore (FAM) and a dark quencher dye.

Instrumentation required: standard real-time PCR instrumentation.

Notes: In addition to being available individually, the Universal ProbeLibrary probes are available in sets (90 probes/set), each of which covers virtually all the transcripts from a single organism. Together, the sets cover approximately 99% of the human, primate, mouse, rat, C. elegans, Drosophila, and Arabidopsis gene transcripts listed in the NCBI Reference Sequence Database.
For more information, see the listings for these organism-specific sets.

For life science research only. Not for use in diagnostic procedures.

LightCycler is a registered trademark of Roche

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Function test: All Universal ProbeLibrary probes are tested for performance in real-time PCR using primers and control templates from the Universal ProbeLibrary Control Set. Each probe must pass this test.

Purity: Each probe is analyzed by anion-exchange HPLC.

Composition: Each probe is analyzed by MALDI-MS.

Use the Universal ProbeLibrary Probes to obtain rapid, flexible quantification of expression levels for virtually any gene from the human, mouse, rat, chimpanzee, C. elegans, Drosophila, or Arabidopsis transcriptomes in the NCBI Reference Sequence Database. Each probe can be used with standard reagents in real-time PCR assays:

• on the LightCycler^® Carousel-Based System (e.g., with the LightCycler^® TaqMan^® Master), or
• on other commercially available real-time PCR instruments (e.g., with the FastStart TaqMan^® Probe Master).

Note: You can easily design gene-specific quantification assays using these probes and the free web-based ProbeFinder software at the Assay Design Center.

Each of the Universal ProbeLibrary probes:

• Significantly reduces assay design time and implementation time.
• Includes features that make probe-based assays specific, flexible, and convenient.
• Needs no special hardware or unusual reaction conditions.

Each Universal ProbeLibrary probe is a single hydrolysis probe that recognizes sequences from one or more of the following organisms: humans, chimpanzees and other primates, mice, rats, C. elegans, Drosophila, Arabidopsis.

Short hydrolysis probes for quantification of gene expression levels by real-time PCR.