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TRANSRTRO

Roche

Transcriptor Reverse Transcriptase

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UNSPSC Code:
41106313
NACRES:
NA.21

recombinant

expressed in E. coli

Quality Level

100

Assay

≥90% (SDS-PAGE)

form

liquid

usage

sufficient for 25 reactions
 sufficient for 50 reactions
 sufficient for 500 reactions

specific activity

0.05 U/mg

feature

dNTPs included: no
hotstart: no

manufacturer/tradename

Roche

packaging

pkg of 200 reactions (03531287001)
pkg of 25 reactions (03531317001)
pkg of 50 reactions (03531295001)

technique(s)

RT-PCR: suitable
RT-qPCR: suitable

color

colorless

pH

7.2

solubility

water: miscible

suitability

suitable for molecular biology

UniProt accession no.

Q9JG39

application(s)

genomic analysis
life science and biopharma

detection method

probe-based

foreign activity

DNase activity, none detected
Nicking activity, none detected
RNase activity, none detected

storage temp.

−20°C (−15°C to −25°C)

Related Categories

General description

A recombinant reverse transcriptase for the robust transcription of RNA fragments up to 14 kb used in two-step RT-PCR on thermal cyclers and real-time PCR instruments.
In retroviruses like the human immunodeficiency virus type 1 (HIV-1), reverse transcriptase (RT) is the core enzyme. HIV-1 RT is made of two subunits of 66 kDa and 51 kDa (p66 and p5l). A human endogenous retrovirus of the HERV-K family codes for reverse transcriptase (RT) enzyme.

Application

Transcriptor Reverse Transcriptase is designed to transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources, using conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler® Instruments) for the following applications:

  • Synthesis of first-strand cDNA for use in subsequent amplification reactions
  • RT-PCR of GC-rich RNA templates
  • Cy3, Cy5, DIG, biotin, and aminoallyl labeling during cDNA synthesis
  • Retrieving and cloning the 5′ and 3′ termini of mRNA by RACE
  • Generation of cDNA libraries with large inserts
  • Dideoxy DNA sequencing
  • RNA sequencing
  • 3′-end labeling of DNA fragments
  • Generation of single-stranded probes for genomic footprints
  • In the reverse transcription of RNA from human Papillomavirus E6, cortical and striatal tissues, muscle biopsies of Becker muscular dystrophy samples and miRNA stem-loop from oocytes and human cerebral microvascular endothelial cells (hCMVECs)

Biochem/physiol Actions

Human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) is essential for the catalytic conversion of single-stranded viral RNA into the double-stranded linear DNA that is integrated into host cell chromosomes.

Features and Benefits

  • Achieve high sensitivity in two-step RT-PCR.
Transcriptor Reverse Transcriptase is used in conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler®Instruments).
  • Obtain more full-length transcripts - up to 14 kb.
cDNA libraries with large inserts can be generated.
  • Reverse transcribe difficult templates.
The enzyme works well at elevated temperatures, thereby overcoming RNA secondary structure (e.g., GC-rich RNA templates) and facilitating optimal reaction conditions.
  • Efficiently label cDNA.
Cy3-, Cy5-, DIG-, biotin-, or aminoallyl-labeled nucleotides are incorporated during cDNA synthesis.

Components

  • Transcriptor Reverse Transcriptase, in storage buffer
  • Transcriptor RT buffer, 5x concentrated

Quality

Each lot of Transcriptor Reverse Transcriptase is routinely function tested in RT-PCR:

  • with a conventional thermal cycler to detect a 10-kb fragment from the human dystrophin gene, starting from human skeletal muscle total RNA
  • with the LightCycler® Instrument to detect 5 x 102 to 5 x 106 copies of in vitro-transcribed human PBGD RNA. The results are defined in fixed crossing points and fixed fluorescence intensity.

Preparation Note

Volume activity: 20 U/μl.
Transcription of long, rare, or difficult RNA targets
Transcriptor Reverse Transcriptase is recommended for RT-PCR of:

  • long targets, because it can transcribe up to 14 kb RNA templates
  • rare targets, because it has high sensitivity
  • GC-rich targets, because it can operate at high temperatures (up to +65oC) to eliminate problems associated with extensive secondary structure

Labeling for many applications
Transcriptor Reverse Transcriptase is also recommended for preparing labeled cDNA, since it accepts a wide variety of modified nucleotides (including Cy3-, Cy5-, DIG-, Biotin-, or aminoallyl-labeled dNTPs).
Reaction requirements
Transcriptor accepts ssRNA and ssDNA templates and requires a primer for transcription.

Storage and Stability

Avoid repeated freezing and thawing.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

LightCycler is a registered trademark of Roche

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tracking disease progression non-invasively in Duchenne and Becker muscular dystrophies
Spitali P, et al.
Journal of Cachexia, Sarcopenia and Muscle, 9, 715-726 (2018)
Valdimara C Vieira et al.
mBio, 5(6) (2014-12-30)
Several recent studies have converged upon the innate immune DNA cytosine deaminase APOBEC3B (A3B) as a significant source of genomic uracil lesions and mutagenesis in multiple human cancers, including those of the breast, head/neck, cervix, bladder, lung, ovary, and other
Identification of an active reverse transcriptase enzyme encoded by a human endogenous HERV-K retrovirus.
Berkhout B, et al.
Journal of Virology, 73(3), 2365-2375 (1999)
Pietro Spitali et al.
Journal of cachexia, sarcopenia and muscle, 9(4), 715-726 (2018-04-24)
Analysis of muscle biopsies allowed to characterize the pathophysiological changes of Duchenne and Becker muscular dystrophies (D/BMD) leading to the clinical phenotype. Muscle tissue is often investigated during interventional dose finding studies to show in situ proof of concept and
Human papillomavirus E6 triggers upregulation of the antiviral and cancer genomic DNA deaminase APOBEC3B
Vieira VC
mBio, 5, e02234-e02214 (2014)
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