TAQNL-RO
Roche
Taq DNA Polymerase (1 U/μl), dNTPack
suitable for PCR, optimum pH ~9.0 (20 °C)
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About This Item
Recommended Products
usage
sufficient for ≤2,000 reactions (04738241001)
sufficient for ≤500 reactions (04738225001)
feature
dNTPs included
hotstart: no
packaging
pkg of 1,000 U (04738241001 [4 x 250 U])
pkg of 250 U (04738225001)
manufacturer/tradename
Roche
parameter
72 °C optimum reaction temp.
technique(s)
PCR: suitable
input
purified DNA
optimum pH
~9.0 (20 °C)
storage temp.
−20°C
General description
Taq DNA Polymerase (1 U/μl), dNTPack, comprises Taq DNA Polymerase and a ready-to-use solution of PCR grade nucleotides. Taq DNA polymerase is a highly processive 5′-3′ DNA polymerase that lacks 3′–5′ exonuclease activity. It is stable during prolonged incubations at elevated temperatures (+95 °C). Additionally, it exhibits highest activity at a pH of around 9 (adjusted at 20 °C) and temperatures around +75 °C. It accepts dNTP analogs as substrates. There is no difference in stability or performance when compared to the standard concentration of Taq DNA Polymerase.
Application
Taq DNA Polymerase (1 U/μl), dNTPack may be used for:
- PCR
- RT-PCR
- Other primer-extension reactions, such as sequencing and labeling
Packaging
1 kit containing 5 components
Unit Definition
One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75 °C under the assay conditions stated under unit assay.
Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Volume Activity: 1 U/μl
Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Volume Activity: 1 U/μl
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- Taq DNA Polymerase
- PCR Buffer with MgCl<sub>2</sub> 10x concentrated
- MgCl<sub>2</sub> Stock Solution
- PCR Buffer without MgCl<sub>2</sub>
- PCR Nucleotide Mix</ul>
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
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Find documentation for the products that you have recently purchased in the Document Library.
Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase.
Y H Zhou et al.
Nucleic acids research, 19(21), 6052-6052 (1991-11-11)
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