T4GENE32-RO
Roche
T4 Gene 32 Protein
from Escherichia coli(infected with phage T4amN134/amBL292/amE218), solution, optimum pH ~8.0
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Synonym(s):
gene 32 protein, t4
UNSPSC Code:
12352202
Recommended Products
biological source
Escherichia coli (infected with phage T4amN134/amBL292/amE218)
Quality Level
form
solution
packaging
pkg of 100 μg (10972983001)
pkg of 500 μg (10972991001)
manufacturer/tradename
Roche
optimum pH
~8.0
storage temp.
−20°C
General description
T4 Gene 32 Protein is reported to improve the yield of long PCR products when 0.5 to 1.0 nmol of the protein is included in the reaction. T4 Gene 32 Protein is also reported to increase the yield of PCR products amplified from samples that contain humic acid. The inhibitory effect of humic acid is reduced by a factor of 7 when T4 Gene 32 Protein is included in the PCR (at a concentration of 2.5 μg/100 μl).
The protein is isolated from the triple-mutant T4amN134/ amBL292/ amE 219 defective for the T4 genes 33, 35, and 58 to 61.
The protein is isolated from the triple-mutant T4amN134/ amBL292/ amE 219 defective for the T4 genes 33, 35, and 58 to 61.
Specificity
T4 gene 32 protein is a single-strand specific, helix-destabilizing protein encoded by gene 32 of the phage T4 genome. It is required for the replication of DNA and genetic recombination in Escherichia coli cells that are infected with T4 bacteriophage.
Application
T4 Gene 32 Protein is a single-strand-specific DNA-binding protein. It may be used for:
- Optimization of PCR (See "Product Description" below.)
- Stimulation of in vitro DNA synthesis
- Stabilization of single-stranded regions of DNA or RNA
- Site-specific mutagenesis experiments (with T4 DNA polymerase and T4 DNA ligase)
- Helping restriction enzyme digestions go to completion
- q-PCR
Quality
Absence of contaminants
T4 gene 32 protein is incubated with various nucleic acids that serve as potential nuclease substrates. Results are analyzed by gel electrophoresis. Based on these tests, none of the following are detectable in the preparation according to the current Quality Control procedures:
T4 gene 32 protein is incubated with various nucleic acids that serve as potential nuclease substrates. Results are analyzed by gel electrophoresis. Based on these tests, none of the following are detectable in the preparation according to the current Quality Control procedures:
- nonspecific exo- and endonucleases
- single-strand DNases (nicking activities)
- single-strand-specific exonucleases
- RNases
Physical form
Protein solution, 1 - 10mg/ml, in storage buffer (20mM Tris-HCl, 100mM NaCl, 1mM EDTA, 0.5mM DTT, 50% glycerol [v/v], pH approximately 8.0)
Preparation Note
Working concentration: 1 to 10 mg/ml
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
No data available
Flash Point(C)
No data available
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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M Bittner et al.
The Journal of biological chemistry, 254(19), 9565-9572 (1979-10-10)
Detailed procedures are presented which allow reproducible preparation of T4 gene 32 protein, a helix-destabilizing protein essential for DNA replication and genetic recombination in T4 bacteriophage-infected Escherichia coli cells. Although 32 protein can be purified to better than 99% homogeneity
Pauline Jacob et al.
International journal of environmental research and public health, 12(3), 2967-2983 (2015-03-13)
A two-year monitoring program of Cryptosporidium parvum oocysts, Giardia duodenalis cysts, Escherichia coli, Clostridium perfringens spores and adenovirus was conducted in three large rivers in France used for recreational activities and as a resource for drinking water production. Fifty-liter river
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