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NADPH-RO

Roche

NADPH

powder, =97% (dry weight)

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Synonym(s):
β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, β-NADPH, 2′-NADPH hydrate, Coenzyme II reduced tetrasodium salt, Dihydronicotinamide adenine dinucleotide phosphate tetrasodium salt, NADPH Na4, TPNH2 Na4, Triphosphopyridine nucleotide reduced tetrasodium salt
Empirical Formula (Hill Notation):
C21H26N7Na4O17P3 · xH2O
CAS Number:
2646-71-1
Molecular Weight:
833.35 (anhydrous basis)
MDL number:
MFCD00036263
UNSPSC Code:
12352200
PubChem Substance ID:
329818701

Quality Level

100

description

Tetrasodium salt

Assay

97% (dry weight)

form

powder

packaging

pkg of 1 g (10621706001)
pkg of 100 mg (10107824001)
pkg of 500 mg (10621692001)

manufacturer/tradename

Roche

λmax

260 and 340 nm

shipped in

wet ice

storage temp.

2-8°C

SMILES string

[Na+].[Na+].[Na+].[Na+].NC(=O)C1=CN(C=CC1)[C@H]2O[C@@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](OP([O-])([O-])=O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@H](O)[C@@H]2O

InChI

1S/C21H30N7O17P3.4Na/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(44-46(33,34)35)14(30)11(43-21)6-41-48(38,39)45-47(36,37)40-5-10-13(29)15(31)20(42-10)27-3-1-2-9(4-27)18(23)32;;;;/h1,3-4,7-8,10-11,13-16,20-21,29-31H,2,5-6H2,(H2,23,32)(H,36,37)(H,38,39)(H2,22,24,25)(H2,33,34,35);;;;/q;4*+1/p-4/t10-,11+,13-,14+,15-,16+,20-,21+;;;;/m0..../s1

InChI key

WYWWVJHQDVCHKF-MPUNMZHWSA-J

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General description

  • Absorbance coefficients: 15.0 x 103 l x mol-1 x cm-1 at 260 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm
  • Absorbance maxima: 260 nm and 340 nm
  • Molecular weight: NADPH-Na4: Mr = 833.4, NADPH: Mr = 745.4
  • pH value in water: 8.0 ± 1.0 (10 mg/ml)
Amorphous powder bottled under nitrogen.

Application

NADPH-RO has been used for the determination of thioredoxin reductase activity.

Biochem/physiol Actions

NADPH is an electron donor and a cofactor for many redox enzymes including nitric oxide synthetase.

Quality

  • Purity: =97% ß-NADPH-Na4 based on dry weight (enzymatically and absorbance at 340 nm), =80% (absorbance at 260 nm)
  • Organic solvents: =3% (GC)

Preparation Note

Storage conditions (working solution): 2 to 8 °C
10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADPH after 4 weeks at 2 to 8 °C.

Analysis Note

Absorbance coefficients: 15.0 x 103 l x mol-1 x cm-1 at 260 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm

Other Notes

For life science research only. Not for use in diagnostic procedures.
Packaged based on solid weight.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Takashi Iyanagi et al.
Archives of biochemistry and biophysics, 528(1), 72-89 (2012-09-18)
NADPH-cytochrome P450 oxidoreductase (CYPOR) and nitric oxide synthase (NOS), two members of the diflavin oxidoreductase family, are multi-domain enzymes containing distinct FAD and FMN domains connected by a flexible hinge. FAD accepts a hydride ion from NADPH, and reduced FAD
Jonna Soerensen et al.
PloS one, 3(3), e1813-e1813 (2008-03-20)
The thioredoxin-dependent system is an essential regulator of cellular redox balance. Since oxidative stress has been linked with neurodegenerative disease, we studied the roles of thioredoxin reductases in brain using mice with nervous system (NS)-specific deletion of cytosolic (Txnrd1) and

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