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LEU-RO

Roche

Leupeptin

powder, from synthetic, =96.5%

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Synonym(s):
Leupeptin hemisulfate salt, Acetyl-Leu-Leu-Arg-al, N-Acetyl-L-leucyl-L-leucyl-L-argininal hemisulfate salt
Linear Formula:
C20H38N6O4 · 1/2H2SO4
CAS Number:
103476-89-7
Molecular Weight:
475.59
MDL number:
MFCD00037012
UNSPSC Code:
12352200
PubChem Substance ID:
329817679

biological source

synthetic

Quality Level

100

description

Ac-Leu-Leu-argininal x 1/2 H2SO4, synthetic

Assay

96.5%

form

powder

mol wt

Mr 475.6

packaging

pkg of 100 mg (11529048001)
pkg of 25 mg (11017128001)
pkg of 5 mg (11017101001)
pkg of 50 mg (11034626001)

manufacturer/tradename

Roche

solubility

H2O: 50 mg/mL

shipped in

wet ice

storage temp.

2-8°C

SMILES string

OS(O)(=O)=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O

InChI

1S/2C20H38N6O4.H2O4S/c2*1-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22;1-5(2,3)4/h2*11-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23);(H2,1,2,3,4)/t2*15-,16-,17-;/m00./s1

InChI key

CIPMKIHUGVGQTG-VFFZMTJFSA-N

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Specificity

Leupeptin inhibits serine and thiol proteases such as trypsin, plasmin, proteinase K, kallikrein, papain, thrombin, and cathepsin A and B.
Not affected are α-, β-, γ- and δ- chymotrypsin, pepsin, cathepsin D, elastase, renin, and thermolysin.

Application

Leupeptin as well as other protease inhibitors like antipain, chymostatin, pepstatin, and phosphoramidon are useful for the protection of proteins during their isolation from tissues or membranes. Leupeptin can be removed from the reaction by dialysis.
Note: To check other protease inhibitors, try our Protease Inhibitor Set including Antipain Dihydrochloride, Aprotinin, Bestatin, Chymostatin, E-64, EDTA-Na2, Leupeptin, Pefabloc SC, Pepstatin, and Phosphoramidon.

Biochem/physiol Actions

Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. There have been numerous studies using leupeptin to protect against hearing loss caused by acoustic overstimulation or the ototoxic antibiotic gentamicin. (Loss of cochlear hair cells is believed to be mediated by calpain.)

Features and Benefits

Contents
Synthetic, white powder

Quality

Function test: Performance controlled with trypsin.

Preparation Note

Working concentration: 0.5 to 5 μg/ml (1 to 20 μM)

Comparison of working concentrations of pefabloc with leupeptin and PMSF is in files.

Working solution: Solvent is recommended in distilled water.
Highly soluble in water (1 mg/ml), methanol, ethanol, acetic acid, dimethyl formamide and dimethyl sulfoxide.
Poorly soluble in acetone, chloroform, ethyl ether and n-hexane.

Storage conditions (working solution): -15 to -25 °C
In aqueous solution leupeptin is stable for 1 month at 2 to 8 °C or for at least 6 months at - 15 to -25 °C, stored under nitrogen. For best results, freeze the dissolved inhibitor in aliquots and avoid repeated thawing.

Reconstitution

Highly soluble in water (1 mg/ml). Stable for at least one week at 2 to 8 °C and 6 months frozen in aliquots at -15 to -25 °C.

Analysis Note

Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined using three main peaks. Majority of contaminating peptide is racemized leupeptin.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302 + H312 + H332

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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