Restriction Protease Factor Xa

Special quality for the cleavage of fusion proteins.
Lyophilizate

Processing of fusion proteins at definite cleavage site. The restriction protease factor Xa is a serine protease which recognizes the amino acid sequence Ile — Glu — Gly — Arg ↓ with a high degree of specificity. This sequence may also be used as a restriction cleavage site for processing of recombinant fusion proteins.

The coagulation factor Xa is a serine protease which recognizes the amino acid sequence Ile—Glu—Gly—Arg ↓ with a high degree of specificity. Cleavage of this sequence activates the natural substrate prothrombin to thrombin. This sequence can also be used as a restriction cleavage site for processing of recombinant fusion proteins. When a lack of specificity is due to steric hindrance, the introduction of an additional amino acid sequence can sometimes overcome the problem.

Working concentration: 1:10 to 1:200 (w/w)
A useable working concentration has a substrate ratio of 1:10 to 1:200 (w/w).

Note: The contents of a vial may be used for several simultaneous digests. When repeating the digest, a new vial should be used. Following this procedure ensures reproducibility and prevents possible contamination.
Storage conditions (working solution): A solution in redistilled water can be used for up to one week when stored at 4 °C.
An aqueous solution, stored in aliquots at -20 °C is stable for approx. one month.
Freezing aliquots increases enzyme stability in solution.

Purity: highly purified (SDS-PAGE); no albumin added
Quality control: function tested
Inhibitors: APMSF, antithrombin III-heparin, DFP, PMSF, soybean trypsin inhibitor

For life science research only. Not for use in diagnostic procedures.