FXA-RO
Roche
Restriction Protease Factor Xa
from bovine plasma
Synonym(s):
factor xa, restriction protease, protease factor xa
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About This Item
Recommended Products
form
lyophilized
mol wt
(Mr = 43 kD)
packaging
pkg of 3 × 100 μg (11585924001)
pkg of 3 × 250 μg (11179896001)
manufacturer/tradename
Roche
optimum pH
8.3
shipped in
wet ice
storage temp.
2-8°C
General description
Special quality for the cleavage of fusion proteins.
Lyophilizate
Lyophilizate
Specificity
The coagulation factor Xa is a serine protease which recognizes the amino acid sequence Ile—Glu—Gly—Arg ↓ with a high degree of specificity. Cleavage of this sequence activates the natural substrate prothrombin to thrombin. This sequence can also be used as a restriction cleavage site for processing of recombinant fusion proteins. When a lack of specificity is due to steric hindrance, the introduction of an additional amino acid sequence can sometimes overcome the problem.
Application
Processing of fusion proteins at definite cleavage site. The restriction protease factor Xa is a serine protease which recognizes the amino acid sequence Ile — Glu — Gly — Arg ↓ with a high degree of specificity. This sequence may also be used as a restriction cleavage site for processing of recombinant fusion proteins.
Quality
Purity: highly purified (SDS-PAGE); no albumin added
Quality control: function tested
Inhibitors: APMSF, antithrombin III-heparin, DFP, PMSF, soybean trypsin inhibitor
Quality control: function tested
Inhibitors: APMSF, antithrombin III-heparin, DFP, PMSF, soybean trypsin inhibitor
Preparation Note
Working concentration: 1:10 to 1:200 (w/w)
A useable working concentration has a substrate ratio of 1:10 to 1:200 (w/w).
Note: The contents of a vial may be used for several simultaneous digests. When repeating the digest, a new vial should be used. Following this procedure ensures reproducibility and prevents possible contamination.
Storage conditions (working solution): A solution in redistilled water can be used for up to one week when stored at 4 °C.
An aqueous solution, stored in aliquots at -20 °C is stable for approx. one month.
Freezing aliquots increases enzyme stability in solution.
A useable working concentration has a substrate ratio of 1:10 to 1:200 (w/w).
Note: The contents of a vial may be used for several simultaneous digests. When repeating the digest, a new vial should be used. Following this procedure ensures reproducibility and prevents possible contamination.
Storage conditions (working solution): A solution in redistilled water can be used for up to one week when stored at 4 °C.
An aqueous solution, stored in aliquots at -20 °C is stable for approx. one month.
Freezing aliquots increases enzyme stability in solution.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(C)
does not flash
Regulatory Information
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