EXPPCRKB
Roche
KAPA Express Extract
with PCR ReadyMix
Synonym(s):
PCR, PCR extraction
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About This Item
UNSPSC Code:
41106300
NACRES:
NA.55
Quality Level
usage
sufficient for 100 reactions
sufficient for 500 reactions
shelf life
≤12 mo.
packaging
pkg of 1000 U (KK7152)
pkg of 200 U (KK7151)
manufacturer/tradename
Roche
technique(s)
DNA extraction: suitable
PCR: suitable
storage temp.
−20°C
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General description
KAPA Express Extract with polymerase chain reaction (PCR) ReadyMix™ is a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA in less than 15 minutes. DNA extractions are conveniently performed in a single-tube thereby greatly reducing the risk of sample loss and contamination.
The combination of KAPA Express Extract and KAPA2G Robust HotStart® ReadyMix™ provides a high performance solution for rapid DNA extraction and consistent downstream amplification.
The combination of KAPA Express Extract and KAPA2G Robust HotStart® ReadyMix™ provides a high performance solution for rapid DNA extraction and consistent downstream amplification.
Application
KAPA Express Extract with PCR ReadyMix™ is ideally suited for the extraction of PCR-ready DNA from, but not limited to, the following sample types:
- Human tissue (formalin-fixed paraffin-embedded (FFPE) samples; blood collected in EDTA tubes or on collection cards; buccal swabs; hair follicles; forensic samples)
- Animal samples (ear or tail clippings; hair follicles; blood; bone marrow; dried or fresh tissue from mouse and other mammals)
- Fish tissues (fin punches; fresh tissue; cold and hot smoked canned samples; ethanol-preserved samples)
- Insects (crushed)
- Bird feathers (calamus fragments).
Features and Benefits
Key Features:
Rapid DNA extraction from a variety of sample types:
Increased PCR success from formalin-fixed paraffin-embedded (FFPE) samples or tissues:
Routine DNA extraction from blood sample types:
Quick Notes:
- Rapid extraction protocol: PCR-ready DNA in 15 minutes
- Versatility: single kit optimized for a variety of sample types
- Single-tube reaction minimizes risk of contamination
- DNA extracted with KAPA Express Extract is compatible with any PCR system
Rapid DNA extraction from a variety of sample types:
- From sample to PCR in less than 15 minutes
- Successful extraction from a variety of tissue types, including buccal swabs, hair follicles, fish tissue, and bird feathers
Increased PCR success from formalin-fixed paraffin-embedded (FFPE) samples or tissues:
- Rapid DNA extraction and consistent downstream amplification of challenging sample types
Routine DNA extraction from blood sample types:
- Efficient extraction from “Guthrie”cards, FTA™ Elute cards, and FTA™ cards
Quick Notes:
- Extract PCR-ready DNA from different tissue types in a quick and simple, single-tube protocol (15–20 min), without the need for hazardous chemicals or multiple washing steps.
- Significantly reduced turnaround times and risk of sample loss and contamination.
- Use 1 μL DNA extract per 25 μL PCR. Depending on the sample type, the extract may have to be diluted prior to PCR.
- One extract typically yields a sufficient amount of template for 50–500 PCRs.
- DNA extracts may be diluted in TE Buffer (1:5) or 10 mM Tris-HCl pH 8–8.5 (1:5–1:10) for long-term storage at -20°C. Extracts are stable at -20°C for ≥6 months.
- KAPA2G Robust HotStart® ReadyMix™ is recommended for consistent and reliable endpoint amplification of DNA extracts generated with KAPA Express Extract.
- No quantification of DNA required prior to PCR.
Quality
All components contained with KAPA Express Extract Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activities, and meet strict requirements with respect to DNA contamination.
Preparation Note
Handling:
KAPA2G Robust HotStart ReadyMix may not freeze solidly, even when stored at -20°C. This will not affect the shelf-life of the product. Always ensure that the product has been fully thawed and mixed before use.
KAPA2G Robust HotStart ReadyMix may not freeze solidly, even when stored at -20°C. This will not affect the shelf-life of the product. Always ensure that the product has been fully thawed and mixed before use.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Legal Information
FTA is a trademark of Qiagen Group
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Kit Components Only
Product No.
Description
- KAPA Express Extract Enzyme 1 U/µL
- 10X KAPA Express Extract Buffer
- 2X KAPA2G Robust HotStart® ReadyMix
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - STOT SE 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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