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CDP-RO

Roche

CDP-Star®

Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.13,7 ]decan}-4-yl)-1-phenyl phosphate

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Synonym(s):
CDP-Star® Chemiluminescent Substrate, ready-to-use, cdp-star, Disodium 2-chloro-5-(4-methoxyspiro[1,2-dioxetane-3,2′-(5-chlorotricyclo[3.3.1.13.7]decan])-4-yl]-1-phenyl phosphate
Empirical Formula (Hill Notation):
C18H19Cl2O7Na2P
CAS Number:
160081-62-9
Molecular Weight:
495.20
MDL number:
MFCD02094611
UNSPSC Code:
12352200
PubChem Substance ID:
329777462

Quality Level

100

Assay

>98%

form

solution

packaging

bottle of 1 mL (11685627001[25 mM])
bottle of 2 × 1 mL (11759051001[25 mM])

manufacturer/tradename

Roche

shipped in

wet ice

storage temp.

2-8°C

SMILES string

Cl[C@]1(C2)C[C@H]3C4(OOC4(C5=CC(OP([O-])([O-])=O)=C(Cl)C=C5)OC)[C@@H]2C[C@@H](C1)C3

InChI

1S/C18H21Cl2O7P.2Na/c1-24-18(14-6-13(2-3-15(14)19)25-28(21,22)23)17(26-27-18)11-4-10-5-12(17)9-16(20,7-10)8-11;;/h2-3,6,10-12H,4-5,7-9H2,1H3,(H2,21,22,23);;/q;2*+1/p-2/t10-,11-,12+,16-,17?,18?;;

InChI key

UKWLRLAKGMZXJC-VCCHNUGISA-L

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General description

Contents
25 mM solution (12.38 mg/ml), 100x concentration

Application

Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:
  • Southern blot
  • Northern blot
  • Dot blot
  • Colony and plaque hybridization
  • Gel shift assays

Features and Benefits

CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems.
  • Save time with a fast, ultra-sensitive substrate.
  • Easily strip and reprobe membranes.
  • Take multiple exposures of up to two days for each experiment.
  • Reuse the substrate by filtering and storing in sodium azide.

Quality

Purity: Purified by HPLC.

Preparation Note

Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable
Maleic Acid Buffer: 15 to 25 °C, stable
Detection Buffer: 15 to 25 °C, stable
Blocking Solution: always prepare freshly
Antibody Solution: 2 to 8 °C

Other Notes

  • First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result.
  • No preincubation step required.
  • Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background).
  • Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
  • Exposure times are shorter than with CSPD.
  • Do not use nitrocellulose membranes.
  • Repeat exposures can be made up to two days after the addition of substrate.
For life science research only. Not for use in diagnostic procedures.

Legal Information

CDP-Star is a registered trademark of Tropix, Inc.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)

No data available

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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