ABTS-RO
Roche
ABTS™ Tablets
tablet, green
Synonym(s):
ABTS
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About This Item
UNSPSC Code:
12352200
form
tablet
packaging
pkg of 20 tablets (11112422001[50mg])
pkg of 20 tablets (11204521001[5mg])
manufacturer/tradename
Roche
color
green
solubility
water: soluble
λmax
414 nm
shipped in
wet ice
storage temp.
2-8°C
General description
ABTS Tablets are used as substrates in enzyme immunoassays with horseradish peroxidase as a marker enzyme. It is widely used in spectrophotometric methods to measure antioxidant activities of food and beverages.
Application
ABTS Tablets are the ideal substrate for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme.
Features and Benefits
- ABTS is soluble in aqueous and organic solvents
- ABTS method is suitable for various pH levels
- Samples react with ABTS quickly and reach stability within 30 minutes
Packaging
- 11204521001 (5 mg/tablet; each tablet is for 5 mL substrate solution)
- 11112422001(50 mg/tablet; each tablet is for 50 mL substrate solution)
Preparation Note
Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of the ABTS buffer solution. This solution has a light-green color. In microtiter plates, it is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.
Analysis Note
Evaluation: photometric (405nm)
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
ABTS is a trademark of Roche
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Haralg Seitz and Sarah Schumacher
Molecular Diagnostics null
R Re et al.
Free radical biology & medicine, 26(9-10), 1231-1237 (1999-06-25)
A method for the screening of antioxidant activity is reported as a decolorization assay applicable to both lipophilic and hydrophilic antioxidants, including flavonoids, hydroxycinnamates, carotenoids, and plasma antioxidants. The pre-formed radical monocation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS*+) is generated by oxidation
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